DTYMK Polyclonal antibody

• Featured Product
• KD/KO Validated

DTYMK Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 15360-1-AP

Synonyms

CDC8, dTMP kinase, DTYMK, Thymidylate kinase, TMPK, TYMK

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Validation Data Gallery

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  WB analysis of HeLa using 15360-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15360-1-AP (DTYMK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 15360-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15360-1-AP (DTYMK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15360-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15360-1-AP (DTYMK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15360-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15360-1-AP (DTYMK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15360-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15360-1-AP (DTYMK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15360-1-AP

  IP result of anti-DTYMK (IP:15360-1-AP, 3ug; Detection:15360-1-AP 1:1000) with HeLa cells lysate 2800ug.

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  IHC staining of human colon cancer using 15360-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15360-1-AP (DTYMK antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 15360-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15360-1-AP (DTYMK antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of MCF-7 using 15360-1-AP

  Immunofluorescent analysis of MCF-7 cells, using DTYMK antibody 15360-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 15360-1-AP

  Immunofluorescent analysis of HepG2 cells, using DTYMK antibody 15360-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  FC experiment of MCF-7 using 15360-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug DTYMK antibody (15360-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, human brain tissue, human heart tissue, mouse brain tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells, HepG2 cells
• Positive FC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below

Product Information

15360-1-AP targets DTYMK in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DTYMK fusion protein Ag7606
• Full Name: deoxythymidylate kinase (thymidylate kinase)
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC001827
• Gene Symbol: DTYMK
• Gene ID (NCBI): 1841
• RRID: AB_2261817
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DTYMK(Thymidylate kinase) is also named as CDC8,TMPK and TYMK.It catalyzes the phosphorylation of dTMP to form dTDP in the dTTP synthesis pathway for DNA synthesis.This enzyme is important in cellular DNA synthesis because the synthesis of dTTP either via the de novo pathway or through the exogenous supply of thymidine requires the activity of this enzyme(PMID:18469).

Publications

Species	Application	Title
human	WB, IHC	Cancer Res; Targeting p300/CBP attenuates hepatocellular carcinoma progression through epigenetic regulation of metabolism.; Authors - Ling-Yan Cai ; KD Validated
human	WB	Cells; Distinct Classes of Flavonoids and Epigallocatechin Gallate, Polyphenol Affects an Oncogenic Mutant p53 Protein, Cell Growth and Invasion in a TNBC Breast Cancer Cell Line.; Authors - Madhu Kollareddy
human	WB	Front Mol Biosci; Deoxythymidylate Kinase as a Promising Marker for Predicting Prognosis and Immune Cell Infiltration of Pan-cancer.; Authors - Tianfeng Lan
human	WB	Proteomes; Utilizing Optimized Tools to Investigate PTM Crosstalk: Identifying Potential PTM Crosstalk of Acetylated Mitochondrial Proteins.; Authors - Henrick Horita
human	WB,IHC	Cell Death Dis; Inhibition of DTYMK significantly restrains the growth of HCC and increases sensitivity to oxaliplatin.; Authors - Fengze Sun ; KD Validated