DUB3 Polyclonal antibody

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• DUB3 Polyclonal antibody (26143-1-AP)

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• KD/KO Validated

DUB3 Polyclonal Antibody for WB, ELISA

Cat No. 26143-1-AP

Publications(11)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, ELISA

Synonyms

Deubiquitinating enzyme 17-like protein 2, deubiquitinating enzyme 3, Deubiquitinating protein 3, DUB 3, DUB-3

• 

  WB analysis using 26143-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26143-1-AP (DUB3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: SGC-7901 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IP: See 3 publications below

Product Information

26143-1-AP targets DUB3 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag23567

  Product name: Recombinant human DUB3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 374-530 aa of BC157073

  Sequence: QKSEWERHSESVSRGREPRALGAEDTDRRATQGELKRDHPCLQAPELDEHLVERATQESTLDHWKFLQEQNKTKPEFNVRKVEGTLPPDVLVIHQSKYKCGMKNHHPEQQSSLLNLSSTTPTHQESMNTGTLASLRGRARRSKGKNKHSKRALLVCQ

• Full Name: deubiquitinating enzyme 3
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC157073
• Gene Symbol: DUB3
• Gene ID (NCBI): 377630
• RRID: AB_2880401
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6R6M4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Phytomedicine; Sarmentosin promotes USP17 and regulates Nrf2-mediated mitophagy and cellular oxidative stress to alleviate APAP-induced acute liver failure.; Authors - Zhitao Jiang
human	WB,IP	Front Pharmacol; Lysophosphatidic Acid-Induced EGFR Transactivation Promotes Gastric Cancer Cell DNA Replication by Stabilizing Geminin in the S Phase.; Authors - Haile Zhao
mouse,human	WB,IHC	Cell Death Dis; CK2α-mediated phosphorylation of DUB3 promotes YAP1 stability and oncogenic functions; Authors - Lei Huang
Human		Anticancer Drugs; NFATc2 promotes lactate and M2 macrophage polarization through USP17 in lung adenocarcinoma; Authors - Liang Wang
human	WB	Adv Sci (Weinh); Redox-Induced Stabilization of AMBRA1 by USP7 Promotes Intestinal Oxidative Stress and Colitis Through Antagonizing DUB3-Mediated NRF2 Deubiquitination; Authors - Weimin Xu
human	WB	iScience; DUB3 is a MAGEA3 deubiquitinase and a potential therapeutic target in hepatocellular carcinoma; Authors - Yuanhong Chen