DUB3 Polyclonal antibody

• Featured Product
• KD/KO Validated

DUB3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 26143-1-AP

Synonyms

deubiquitinating enzyme 3, Deubiquitinating protein 3, DUB 3, DUB3, USP17L2

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Validation Data Gallery

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  WB analysis using 26143-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26143-1-AP (DUB3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: SGC-7901 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IP: See 2 publications below

Product Information

The immunogen of 26143-1-AP is DUB3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DUB3 fusion protein Ag23567
• Full Name: deubiquitinating enzyme 3
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC157073
• Gene Symbol: DUB3
• Gene ID (NCBI): 377630
• RRID: AB_2880401
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Phytomedicine; Sarmentosin promotes USP17 and regulates Nrf2-mediated mitophagy and cellular oxidative stress to alleviate APAP-induced acute liver failure.; Authors - Zhitao Jiang
human	WB,IP	Front Pharmacol; Lysophosphatidic Acid-Induced EGFR Transactivation Promotes Gastric Cancer Cell DNA Replication by Stabilizing Geminin in the S Phase.; Authors - Haile Zhao
human	WB,IP	Breast Cancer Res Treat; USP17L2-SIRT7 axis regulates DNA damage repair and chemoresistance in breast cancer cells; Authors - Yang Su ; KD Validated
human	WB	Oncogene; C4orf19 inhibits colorectal cancer cell proliferation by competitively binding to Keap1 with TRIM25 via the USP17/Elk-1/CDK6 axis; Authors - Shirui Huang
human	WB	iScience; DUB3 is a MAGEA3 deubiquitinase and a potential therapeutic target in hepatocellular carcinoma; Authors - Yuanhong Chen
mouse	WB	FASEB J; Melatonin alleviates hyperoxia-induced lung injury through elevating MSC exosomal miR-18a-5p expression to repress PUM2 signaling; Authors - Dongmei Zou