DUB3 Polyclonal antibody

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• KD/KO Validated

DUB3 Polyclonal Antibody for WB, ELISA

Cat No. 26143-1-AP Copied!

Publications (11)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, ELISA

Synonyms

Deubiquitinating enzyme 17-like protein 2, deubiquitinating enzyme 3, Deubiquitinating protein 3, DUB 3, DUB-3

• 

  WB analysis using 26143-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26143-1-AP (DUB3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: SGC-7901 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26143-1-AP targets DUB3 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag23567

  Product name: Recombinant human DUB3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 374-530 aa of BC157073

  Sequence: QKSEWERHSESVSRGREPRALGAEDTDRRATQGELKRDHPCLQAPELDEHLVERATQESTLDHWKFLQEQNKTKPEFNVRKVEGTLPPDVLVIHQSKYKCGMKNHHPEQQSSLLNLSSTTPTHQESMNTGTLASLRGRARRSKGKNKHSKRALLVCQ

• Full Name: deubiquitinating enzyme 3
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC157073
• Gene Symbol: DUB3
• Gene ID (NCBI): 377630
• RRID: AB_2880401
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6R6M4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The antibody targeting USP17 (Cat# 26143-1-AP) has 11 citations across journals including MedComm, Advanced Science, Cell Death & Disease, Oncogene, Cell Death Discovery, iScience, FASEB Journal, Phytomedicine, Frontiers in Pharmacology, Breast Cancer Research and Treatment, and Anticancer Drugs. Research fields span oncology (colorectal, gastric, liver, breast, and lung cancers), oxidative stress, mitophagy, DNA damage repair, and immune cell polarization.

Automatically generated from the 11 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	MedComm (2020); Targeting CDK4/6 suppresses colorectal cancer by destabilizing YAP1; Authors - Yalei Wen
human	WB	Adv Sci (Weinh); Redox-Induced Stabilization of AMBRA1 by USP7 Promotes Intestinal Oxidative Stress and Colitis Through Antagonizing DUB3-Mediated NRF2 Deubiquitination; Authors - Weimin Xu
mouse,human	WB,IHC	Cell Death Dis; CK2α-mediated phosphorylation of DUB3 promotes YAP1 stability and oncogenic functions; Authors - Lei Huang
human	WB	Phytomedicine; Sarmentosin promotes USP17 and regulates Nrf2-mediated mitophagy and cellular oxidative stress to alleviate APAP-induced acute liver failure.; Authors - Zhitao Jiang
human	WB,IP	Cell Death Discov; CDK4/6-mediated phosphorylation of DUB3 promotes YAP1 stability and hepatocellular carcinoma progression; Authors - Lei Huang
human	WB	Oncogene; C4orf19 inhibits colorectal cancer cell proliferation by competitively binding to Keap1 with TRIM25 via the USP17/Elk-1/CDK6 axis; Authors - Shirui Huang