DUOXA1 Polyclonal antibody

• Featured Product
• KD/KO Validated

DUOXA1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 26632-1-AP

Synonyms

Dual oxidase activator 1, DUOXA1, mol, NIP, Numb interacting protein, NUMBIP

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Validation Data Gallery

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  WB analysis of mouse colon using 26632-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 26632-1-AP (DUOXA1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 26632-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26632-1-AP (DUOXA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 26632-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26632-1-AP (DUOXA1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse colon tissue, Jurkat cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

The immunogen of 26632-1-AP is DUOXA1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DUOXA1 fusion protein Ag24373
• Full Name: dual oxidase maturation factor 1
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 29 kDa, 52 kDa
• GenBank Accession Number: BC020841
• Gene Symbol: DUOXA1
• Gene ID (NCBI): 90527
• RRID: AB_2880581
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DUOXA1, also named as NIP and NUMBIP, originally identified as a Numb-interacting protein, was recently shown to function as a maturation factor for the dual oxidase 1(DUOX1). DUOXA1 transient overexpression affected the cell-cell adhesion by modulating the actin cytoskeleton, and sensitized cells to doxorubicin. DUOXA1 has some isoforms with MW 33 kDa, 38 kDa and 54 kDa.

Publications

Species	Application	Title
human	WB	Oncogene; PMID: 30166592; Authors - Jing Sun ; KD Validated