Tested Applications
| Positive WB detected in | mouse colon tissue, Jurkat cells, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26632-1-AP targets DUOXA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24373 Product name: Recombinant human DUOXA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 162-298 aa of BC020841 Sequence: GYMLLATGIFQLLALLFFSMATSLTSPCPLHLGASVLHTHHGPAFWITLTTGLLCVLLGLAMAVAHRMQPHRLKAFFNQSVDEDPMLEWSPEEGGLLSPRYRSMADSPKSQDIPLSEASSTKAYCKEAHPKDPDCAL Predict reactive species |
| Full Name | dual oxidase maturation factor 1 |
| Calculated Molecular Weight | 38 kDa |
| Observed Molecular Weight | 29 kDa, 52 kDa |
| GenBank Accession Number | BC020841 |
| Gene Symbol | DUOXA1 |
| Gene ID (NCBI) | 90527 |
| RRID | AB_2880581 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q1HG43 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DUOXA1, also named as NIP and NUMBIP, originally identified as a Numb-interacting protein, was recently shown to function as a maturation factor for the dual oxidase 1(DUOX1). DUOXA1 transient overexpression affected the cell-cell adhesion by modulating the actin cytoskeleton, and sensitized cells to doxorubicin. DUOXA1 has some isoforms with MW 33 kDa, 38 kDa and 54 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DUOXA1 antibody 26632-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DUOXA1 Polyclonal antibody (Cat# 26632-1-AP) has 3 total citations. These appear in Redox Biology, Oncogene, and FASEB Journal. The cited research spans prostate cancer (DNMT1-DUOXA1 methylation-ferroptosis axis in bicalutamide resistance), ovarian cancer (miR-137/c-Myc/EZH2 cisplatin resistance), and asthma (DECR1-targeted ferroptosis and inflammation resolution in airway epithelial cells). All citations involve human samples, with applications in WB and IHC.
| Species | Application | Title |
|---|---|---|
Redox Biol DNMT1-DUOXA1 axis discovers a novel methylation-ferroptosis circuit in bicalutamide-resistant prostate cancer.
| ||
FASEB J Targeting DECR1 Mitigates Ferroptosis in Airway Epithelial Cells and Promotes Inflammation Resolution in Asthma. |
Reviews
What customers say
Two reviews were submitted for this antibody, both for Western Blot applications. One user (4/5) reported successful results on mouse lung homogenate at 1:20 dilution using the SimpleWestern protocol. Another user (2/5) tested it on A549 cells at 1:1000 dilution and found the antibody lacked specificity for DUOXA1. Overall, feedback is mixed: the antibody performed well in one tissue-based application but showed non-specific binding in a cell line context.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Anis (Verified Customer) (06-26-2025) | Jess - SimpleWestern mouse lunge homogenate 1:20 dilution in antibody diluent 2
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Edma (Verified Customer) (03-03-2025) | The antibody not specific for the duoxa1
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