Tested Applications
| Positive WB detected in | A431 cells, HeLa cells, K-562 cells |
| Positive IP detected in | K-562 cells |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:700-1:2800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10204-2-AP targets DUSP11 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0276 Product name: Recombinant human DUSP11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-203 aa of BC000346 Sequence: MSQWHHPRSGWGRRRDFSGRSSAKKKGGNHIPERWKDYLPVGQRMPGTRFIAFKVPLQKSFEKKLAPEECFSPLDLFNKIREQNEELGLIIDLTYTQRYYKPEDLPETVPYLKIFTVGHQVPDDETIFKFKHAVNGFLKENKDNDKLIGVHCTHGLNRTGYLICIYLIDVEGVRPDDAIELFNRCRGHCLERQNYIEDLQNGP Predict reactive species |
| Full Name | dual specificity phosphatase 11 (RNA/RNP complex 1-interacting) |
| Calculated Molecular Weight | 39 kDa |
| Observed Molecular Weight | 39 kDa |
| GenBank Accession Number | BC000346 |
| Gene Symbol | DUSP11 |
| Gene ID (NCBI) | 8446 |
| RRID | AB_2277484 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75319 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DUSP11, also known as PIR1, is a unique member of atypical DUSPs which could bind directly to RNA and possess RNA 5'-triphosphatase and diphosphatase activities. DUSP11 converts the 5' triphosphate of microRNA precursors to a 5' monophosphate, and regulates cellular noncoding RNAs levels. In addition to a catalysis towards RNA, more evidence showed that DUSP11 could also dephosphorylate proteins.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DUSP11 antibody 10204-2-AP | Download protocol |
| IP protocol for DUSP11 antibody 10204-2-AP | Download protocol |
| WB protocol for DUSP11 antibody 10204-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-DUSP11 antibody (Cat# 10204-2-AP) has 12 citations across journals including Nature Communications, PNAS, Nucleic Acids Research, Genes & Development, Annals of the Rheumatic Diseases, Cancer Immunology Research, Cellular and Molecular Life Sciences, iScience, RNA Biology, Journal of Cellular and Molecular Medicine, Virologica Sinica, and bioRxiv. Research fields span RNA biology, innate immunity, viral infection (HCV, KSHV, HIV-1, HBV), cancer (lung adenocarcinoma, hepatocellular carcinoma), and rheumatoid arthritis.
| Species | Application | Title |
|---|---|---|
Nat Commun RIG-I like receptor sensing of host RNAs facilitates the cell-intrinsic immune response to KSHV infection. | ||
Nucleic Acids Res DUSP11 is an RNA triphosphatase that limits PspCas13b activity by destabilizing gRNA abundance in mammalian cells.
| ||
Proc Natl Acad Sci U S A RNA triphosphatase DUSP11 enables exonuclease XRN-mediated restriction of hepatitis C virus.
| ||
Cancer Immunol Res DUSP11 is an Intracellular Innate Immune Checkpoint in Lung Adenocarcinoma.
| ||
Genes Dev DUSP11 activity on triphosphorylated transcripts promotes Argonaute association with noncanonical viral microRNAs and regulates steady-state levels of cellular noncoding RNAs. |
