DUSP11 Polyclonal antibody

• Featured Product
• KD/KO Validated

DUSP11 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 10204-2-AP

Synonyms

DUSP11, PIR1

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Validation Data Gallery

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  WB analysis using 10204-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10204-2-AP (DUSP11 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 10204-2-AP

  IP result of anti-DUSP11 (IP:10204-2-AP, 4ug; Detection:10204-2-AP 1:4000) with K-562 cells lysate 1760 ug.

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  IF Staining of A431 using 10204-2-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using DUSP11 antibody (10204-2-AP) at dilution of 1:1400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, K-562 cells
• Positive IP detected in: K-562 cells
• Positive IF detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:700-1:2800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below

Product Information

10204-2-AP targets DUSP11 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DUSP11 fusion protein Ag0276
• Full Name: dual specificity phosphatase 11 (RNA/RNP complex 1-interacting)
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC000346
• Gene Symbol: DUSP11
• Gene ID (NCBI): 8446
• RRID: AB_2277484
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DUSP11, also known as PIR1, is a unique member of atypical DUSPs which could bind directly to RNA and possess RNA 5'-triphosphatase and diphosphatase activities. DUSP11 converts the 5' triphosphate of microRNA precursors to a 5' monophosphate, and regulates cellular noncoding RNAs levels. In addition to a catalysis towards RNA, more evidence showed that DUSP11 could also dephosphorylate proteins.

Publications

Species	Application	Title
human	WB	Ann Rheum Dis; Novel autoantibodies identified in ACPA-negative rheumatoid arthritis.; Authors - Ketian Li
human	WB	Nat Commun; RIG-I like receptor sensing of host RNAs facilitates the cell-intrinsic immune response to KSHV infection.; Authors - Yang Zhao
human	WB	Proc Natl Acad Sci U S A; RNA triphosphatase DUSP11 enables exonuclease XRN-mediated restriction of hepatitis C virus.; Authors - Rodney P Kincaid ; KO Validated
human	WB	Genes Dev; DUSP11 activity on triphosphorylated transcripts promotes Argonaute association with noncanonical viral microRNAs and regulates steady-state levels of cellular noncoding RNAs.; Authors - James M Burke
human	WB	Virol Sin; HBV precore G1896A mutation promotes growth of hepatocellular carcinoma cells by activating ERK/MAPK pathway; Authors - Baoxin Zhao ; KD Validated
human	WB	RNA Biol; DUSP11 - an RNA phosphatase that regulates host and viral non-coding RNAs in mammalian cells.; Authors - James M Burke