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DUSP12 Polyclonal antibody

DUSP12 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15667-1-AP

Synonyms

DUSP12, YVH1



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inmouse brain tissue, mouse embryo tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15667-1-AP targets DUSP12 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DUSP12 fusion protein Ag8131
Full Name dual specificity phosphatase 12
Calculated Molecular Weight 370 aa, 43 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC006286
Gene Symbol DUSP12
Gene ID (NCBI) 11266
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DUSP12 (Dual specificity protein phosphatase 12) belongs to the protein-tyrosine phosphatase family and non-receptor class dual specificity subfamily. DUSP12 is a kind of dual specificity phosphatase. It can dephosphorylate both phosphotyrosine and phosphoserine or phosphothreonine residues and can dephosphorylate glucokinase in vitro (PMID:10446167). In addition, DUSP12 has phosphatase activity with the synthetic substrate 6,8-difluoro-4-methylumbelliferyl phosphate and other in vitro substrates (PMID:24531476). DUSP12 is ubiquitously expressed in spleen, testis, ovary, and peripheral blood leukocytes and lower expression in liver and lung.

Protocols

Product Specific Protocols
WB protocol for DUSP12 antibody 15667-1-APDownload protocol
IHC protocol for DUSP12 antibody 15667-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols