Tested Applications
| Positive WB detected in | mouse testis tissue, human skeletal muscle tissue, rat testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human colon cancer tissue, rat skeletal muscle tissue, human colon cancer tissue, human thyroid cancer tissue, mouse skeletal muscle tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10909-1-AP targets DUSP13 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1348 Product name: Recombinant human DUSP13 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-198 aa of BC009778 Sequence: MDSLQKQDLRRPKIHGAVQASPYQPPTLASLQRLLWVRQAATLNHIDEVWPSLFLGDAYAARDKSKLIQLGITHVVNAAAGKFQVDTGAKFYRGMSLEYYGIEADDNPFFDLSVYFLPVARYIRAALSVPQGRVLVHCAMGVSRSATLVLAFLMIYENMTLVEAIQTVQAHRNICPNSGFLRQLQVLDNRLGRETGRF Predict reactive species |
| Full Name | dual specificity phosphatase 13 |
| Calculated Molecular Weight | 22 kDa, 36 kDa |
| Observed Molecular Weight | 22 kDa, 36 kDa |
| GenBank Accession Number | BC009778 |
| Gene Symbol | DUSP13 |
| Gene ID (NCBI) | 51207 |
| RRID | AB_2230734 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UII6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DUSP13 antibody 10909-1-AP | Download protocol |
| IP protocol for DUSP13 antibody 10909-1-AP | Download protocol |
| WB protocol for DUSP13 antibody 10909-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DUSP13 Polyclonal antibody (catalog 10909-1-AP) has 3 citations. They appear in Biomedicines, Fundam Res, and Transl Androl Urol. Research fields include p53/DUSP13 isoform synthesis, C3aR/STAT3 signaling in neuroinflammation and neuromyelitis optica, and DUSP13-driven prostate cancer progression via the ERK1/2 pathway.
| Species | Application | Title |
|---|---|---|
Biomedicines The Strong Activation of p53 Tumor Suppressor Drives the Synthesis of the Enigmatic Isoform of DUSP13 Protein | ||
Fundam Res Targeting C3aR/STAT3 signaling attenuates neuroinflammation and reverses neurological impairment in Neuromyelitis optica. | ||
Transl Androl Urol DUSP13 promotes prostate cancer progression by regulating the ERK1/2 pathway. |
Reviews
What customers say
The single review for this product is a 5-star rating from a Western Blot application. The reviewer reported that the antibody detected the target at the expected molecular weight, using a 1:3000 dilution. Overall, the feedback is positive, confirming reliable performance for WB.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Agnieszka (Verified Customer) (01-13-2023) | Product observed at the correct height
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