DUSP2 Polyclonal antibody

DUSP2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 27327-1-AP

Synonyms

dual specificity phosphatase 2, DUSP2, PAC 1, PAC1

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Validation Data Gallery

• 

  WB analysis of A549 using 27327-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27327-1-AP (DUSP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27327-1-AP (DUSP2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 27327-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27327-1-AP (DUSP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• FC: See 1 publications below

Product Information

27327-1-AP targets DUSP2 in WB, FC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DUSP2 fusion protein Ag25380
• Full Name: dual specificity phosphatase 2
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC007771
• Gene Symbol: DUSP2
• Gene ID (NCBI): 1844
• RRID: AB_2880845
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DUSP2 belongs to the sub-family of nuclear specific DUSPs that includes DUSP1, 4 and 5. DUSP2 is highly expressed in immune cells, in particular, of patients with inflammatory arthritis, such as in rheumatoid arthritis (RA), and Dusp2 knockout mice present a significant reduction in inflammatory responses. It has been demonstrated that DUSP2 is a downstream target of the tumor suppressor p53 that is required for the cellular response to oxidative stress. DUSP2 is also involved in transcription factor E2F1-mediated apoptotic signaling. Like other members of the DUSP family, DUSP2 interacts with MAPKs; However, the target protein for its phosphatase activity is controversial.

Publications

Species	Application	Title
human	WB	Hum Cell; Exosomal hsa_circ_0017252 attenuates the development of gastric cancer via inhibiting macrophage M2 polarization.; Authors - Jin Song
mouse	WB	Basic Clin Pharmacol Toxicol; Apatinib combined with Keytruda treatment induces apoptosis of gastric carcinoma cells through CES4/miR-616-5p/DUSP2 axis.; Authors - Fengli Zhang
human	FC	Blood Coagul Fibrinolysis; Effects of Momordica charantia exosomes on platelet activation, adhesion, and aggregation.; Authors - Jianchao Zhang
mouse	WB	Bioact Mater; Large extracellular vesicles secreted by human iPSC-derived MSCs ameliorate tendinopathy via regulating macrophage heterogeneity; Authors - Teng Ye
mouse	WB	Cell Rep; GABA regulates IL-1β production in macrophages; Authors - Jian Fu ; KD Validated