MKP-2/DUSP4 Polyclonal antibody

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  WB analysis using 10739-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10739-1-AP (MKP-2/DUSP4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of MDA-MB-231 using 10739-1-AP

  IP result of anti-MKP-2/DUSP4 (IP:10739-1-AP, 4ug; Detection:10739-1-AP 1:2000) with MDA-MB-231 cells lysate 1280 ug.

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  FC experiment of MDA-MB-231 using 10739-1-AP

  1x10^6 MDA-MB-231 cells were intracellularly stained with 0.25 ug MKP-2/DUSP4 Polyclonal antibody (10739-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• Featured Product
• KD/KO Validated

MKP-2/DUSP4 Polyclonal Antibody for WB, IP, ELISA

Cat No. 10739-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Synonyms

DUSP4, MKP-2, dual specificity phosphatase 4, EC:3.1.3.16, EC:3.1.3.48

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Tested Applications

• Positive WB detected in: HCT 116 cells, SK-BR-3 cells
• Positive IP detected in: MDA-MB-231 cells
• Positive FC (Intra) detected in: MDA-MB-231 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

10739-1-AP targets MKP-2/DUSP4 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MKP-2/DUSP4 fusion protein Ag1206
• Full Name: dual specificity phosphatase 4
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 40-43 kDa
• GenBank Accession Number: BC014565
• Gene Symbol: DUSP4
• Gene ID (NCBI): 1846
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13115
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DUSP4, also named as MKP-2, is a member of the mitogen-activated protein (MAP) kinase phosphatase family which has been suggested to play an important role in the feedback control of MAP kinase-mediated gene expression. DUSP4 preferentially inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus.

Publications

Species	Application	Title
mouse	WB,IHC	J Clin Invest; Compound haploinsufficiency of Dok2 and Dusp4 promotes lung tumorigenesis.; Authors - Ming Chen ; KO Validated
mouse	WB	Cell Death Discov; NR4A1 enhances MKP7 expression to diminish JNK activation induced by ROS or ER-stress in pancreatic β cells for surviving.; Authors - Ze-Qing Pu