DUSP9 Polyclonal antibody

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  WB analysis using 26718-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26718-1-AP (DUSP9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26718-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26718-1-AP (DUSP9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 26718-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26718-1-AP (DUSP9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 26718-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26718-1-AP (DUSP9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DUSP9 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 26718-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

dual specificity phosphatase 9, EC:3.1.3.16, MAP kinase phosphatase 4, Mitogen-activated protein kinase phosphatase 4, MKP 4

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

26718-1-AP targets DUSP9 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DUSP9 fusion protein Ag25167
• Full Name: dual specificity phosphatase 9
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC060837
• Gene Symbol: DUSP9
• Gene ID (NCBI): 1852
• RRID: AB_2880612
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99956
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dual specificity protein phosphatase 9 (DUSP9) is also named itogen-activated protein kinase phosphatase 4 (MAP kinase phosphatase 4; MKP-4). DUSP9 is a typical DUSP characterized by the presence of an MKB/KIM motif and a phosphatase domain, which shares structural homology with other DUSPs (PMID: 25519881). DUSP9 is an important downstream regulator of BMP/Smad signaling and a key factor in maintaining the stemness of mESCs (PMID: 34768967). DUSP9 is one of the key genes involved in gonadotrophin-mediated ovarian follicle development (PMID: 33676987). DUSP9 expression increases during insulin-induced adipogenesis and culminates in mature adipocytes. DUSP9 as a key regulator of insulin signaling and highlighted its potential role in insulin resistance and metabolic diseases by dephosphorylating kinases involved in metabolic processes, glucose uptake and storage. DUSP9 can also impair the action of extracellular mediators and stress inducers (i.e., proinflammatory cytokines), which can induce insulin resistance by abnormally activating MAPK or SAP pathways (PMID: 30063256, PMID: 18296638). In normal mature tissues, DUSP9 is mainly expressed in kidney, adipose tissue and placenta, while it is only minimally present in brain, ovary, testis and urinary bladder (PMID: 34768967).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Front Immunol; Identifying specific TLS-associated genes as potential biomarkers for predicting prognosis and evaluating the efficacy of immunotherapy in soft tissue sarcoma; Authors - Xiang-Xu Wang
mouse	WB	Sci Rep; Inhibition of miR-194-5p avoids DUSP9 downregulation thus limiting sepsis-induced cardiomyopathy; Authors - Jie Wang ; KD Validated
human	WB,IF	Adv Sci (Weinh); Malignant Hepatoblast-Like Cells Sustain Stemness via IGF2-Dependent Cholesterol Accumulation in Hepatoblastoma; Authors - Miao Ding