DUSP9 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis of HEK-293 using 86199-3-RR (same clone as 86199-3-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 86199-3-RR (DUSP9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86199-3-PBS in a different storage buffer formulation.

• 

  WB analysis using 86199-3-RR (same clone as 86199-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86199-3-RR (DUSP9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86199-3-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 86199-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86199-3-RR against Human DUSP9 were performed. The affinity constant is 3.30 nM.

DUSP9 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 86199-3-PBS

Clone No.250824B11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

dual specificity phosphatase 9, EC:3.1.3.16, MAP kinase phosphatase 4, Mitogen-activated protein kinase phosphatase 4, MKP 4

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Product Information

86199-3-PBS targets DUSP9 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag25167

  Product name: Recombinant human DUSP9 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 343-384 aa of BC060837

  Sequence: SLRLEERHSQEQGSGGQASAASNPPSFFTTPTSDGAFELAPT

• Full Name: dual specificity phosphatase 9
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC060837
• Gene Symbol: DUSP9
• Gene ID (NCBI): 1852
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q99956
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Dual specificity protein phosphatase 9 (DUSP9) is also named itogen-activated protein kinase phosphatase 4 (MAP kinase phosphatase 4; MKP-4). DUSP9 is a typical DUSP characterized by the presence of an MKB/KIM motif and a phosphatase domain, which shares structural homology with other DUSPs (PMID: 25519881). DUSP9 is an important downstream regulator of BMP/Smad signaling and a key factor in maintaining the stemness of mESCs (PMID: 34768967). DUSP9 is one of the key genes involved in gonadotrophin-mediated ovarian follicle development (PMID: 33676987). DUSP9 expression increases during insulin-induced adipogenesis and culminates in mature adipocytes. DUSP9 as a key regulator of insulin signaling and highlighted its potential role in insulin resistance and metabolic diseases by dephosphorylating kinases involved in metabolic processes, glucose uptake and storage. DUSP9 can also impair the action of extracellular mediators and stress inducers (i.e., proinflammatory cytokines), which can induce insulin resistance by abnormally activating MAPK or SAP pathways (PMID: 30063256, PMID: 18296638). In normal mature tissues, DUSP9 is mainly expressed in kidney, adipose tissue and placenta, while it is only minimally present in brain, ovary, testis and urinary bladder (PMID: 34768967).