DVL2 Monoclonal antibody, PBS Only

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  WB analysis using 67105-1-Ig (same clone as 67105-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67105-1-Ig (DVL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 67105-1-Ig (same clone as 67105-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67105-1-Ig (same clone as 67105-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67105-1-Ig (DVL2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.

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  WB analysis of T-47D using 67105-1-Ig (same clone as 67105-1-PBS)

  T-47D cells were subjected to SDS PAGE followed by western blot with 67105-1-Ig (DVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 67105-1-Ig (same clone as 67105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 67105-1-Ig (same clone as 67105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.

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  IHC staining of rat colon using 67105-1-Ig (same clone as 67105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.

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  IHC staining of rat colon using 67105-1-Ig (same clone as 67105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 67105-1-Ig (same clone as 67105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 67105-1-Ig (same clone as 67105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67105-1-Ig (same clone as 67105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67105-1-Ig (same clone as 67105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 67105-1-Ig (same clone as 67105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 67105-1-Ig (same clone as 67105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.

DVL2 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 67105-1-PBS

Clone No.2F2D2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Dishevelled 2, 2F2D2, Dishevelled-2, DSH homolog 2, Segment polarity protein dishevelled homolog DVL-2

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Product Information

67105-1-PBS targets DVL2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28634

  Product name: Recombinant human DVL2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-340 aa of BC014844

  Sequence: MAGSSTGGGGVGETKVIYHLDEEETPYLVKIPVPAERITLGDFKSVLQRPAGAKYFFKSMDQDFGVVKEEISDDNARLPCFNGRVVSWLVSSDNPQPEMAPPVHEPRAELAPPAPPLPPLPPERTSGIGDSRPPSFHPNVSSSHENLEPETETESVVSLRRERPRRRDSSEHGAGGHRTGGPSRLERHLAGYESSSTLMTSELESTSLGDSDEEDTMSRFSSSTEQSSASRLLKRHRRRRKQRPPRLERTSSFSSVTDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDMNFENMSNDDAVRVLRDI

• Full Name: dishevelled, dsh homolog 2 (Drosophila)
• Calculated Molecular Weight: 736 aa, 79 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC014844
• Gene Symbol: DVL2
• Gene ID (NCBI): 1856
• RRID: AB_2882409
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O14641
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.