DYKDDDDK tag Recombinant antibody, PBS Only (Binds to FLAG® tag epitope)

DYKDDDDK tag Recombinant Antibody for IP, IF, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

IP, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4K14

Cat no : 80010-1-PBS

Synonyms

DDDDK, DDDDK tag, DYKDDDDK, DYKDDDDK tag, Flag, Flag tag

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Validation Data Gallery

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  IP experiment of Transfected HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

  IP result of anti-DYKDDDDK tag (IP:80010-1-RR, 2.5 ug; WB:66008-3-Ig, 1:3000) with Transfect HEK-293 cells lysate 768 ug. This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 (eGFP-DYKDDDDK transfected) cells using DYKDDDDK tag antibody (80010-1-RR, Clone: 4K14 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), eGFP (Green), DYKDDDDK (Red), DAPI (Blue). This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Transfected HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using 80010-1-RR (DDDDK tag antibody), at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Transfected HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using 80010-1-RR (DYKDDDDK tag antibody), at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using DYKDDDDK tag antibody (80010-1-RR, Clone: 4K14 ) at dilution of 1:500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), GFP (green). This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80010-1-PBS targets DYKDDDDK tag in IP, IF, Indirect ELISA applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DYKDDDDK tag fusion protein Ag2329
• Full Name: DYKDDDDK tag
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

DYKDDDDK Tag (Equivalent To FLAG Antibody From Sigma) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.