Dymeclin Polyclonal antibody

Dymeclin Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 12001-1-AP

Synonyms

DMC, DYM, Dymeclin, SMC

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Validation Data Gallery

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  WB analysis using 12001-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12001-1-AP (Dymeclin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, MCF-7 cells, mouse kidney tissue, mouse pancreas tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12001-1-AP targets Dymeclin in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Dymeclin fusion protein Ag2600
• Full Name: dymeclin
• Calculated Molecular Weight: 669 aa, 76 kDa
• Observed Molecular Weight: 76-80 kDa
• GenBank Accession Number: BC001252
• Gene Symbol: DYM
• Gene ID (NCBI): 54808
• RRID: AB_2246056
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human Dymeclin is encoded by DYM gene, defects in which are the cause of Dyggve-Melchior-Clausen syndrome (DMC) and Smith-McCort dysplasia (SMC). DMC is a rare autosomal recessive disorder characterized by short trunk dwarfism, microcephaly and psychomotor retardation, with cutaneous cells containing dilated rough endoplasmic reticulum, enlarged and aberrant vacuoles and numerous vesicles [PubMed: 12491225]. Distinct with features of DMS, SMC is a rare osteochondrodysplasia characterized by short limbs and trunk with barrel-shaped chest [PubMed: 19005420]. Dymeclin is a not a stably anchored transmembrane protein with a transmembrane domain, but it shuttles between Golgi and cytosol, which is necessary for correct organization of Golgi apparatus [PubMed: 18996921].