Tested Applications
| Positive WB detected in | HEK-293 cells, MCF-7 cells, mouse kidney tissue, mouse pancreas tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12001-1-AP targets Dymeclin in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2600 Product name: Recombinant human DYM protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 242-590 aa of BC001252 Sequence: PSSIPHAFQINFNSLYTALCEQQTSDQATLLLYTLLHQNSNIRTYMLARTDMENLVLPILEILYHVEERNSHHVYMALIILLILTEDDGFNRSIHEVILKNITWYSERVLTEISLGSLLILVVIRTIQYNMTRTRDKYLHTNCLAALANMSAQFRSLHQYAAQRIISLFSLLSKKHNKVLEQATQSLRGSLSSNDVPLPDYAQDLNVIEEVIRMMLEIINSCLTNSLHHNPNLVYALLYKRDLFEQFRTHPSFQDIMQNIDLVISFFSSRLLQAGAELSVERVLEIIKQGVVALPKDRLKKFPELKFKYVEEEQPEEFFIPYVWSLVYNSAVGLYWNPQDIQLFTMDSD Predict reactive species |
| Full Name | dymeclin |
| Calculated Molecular Weight | 669 aa, 76 kDa |
| Observed Molecular Weight | 76-80 kDa |
| GenBank Accession Number | BC001252 |
| Gene Symbol | Dymeclin |
| Gene ID (NCBI) | 54808 |
| RRID | AB_2246056 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7RTS9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Human Dymeclin is encoded by DYM gene, defects in which are the cause of Dyggve-Melchior-Clausen syndrome (DMC) and Smith-McCort dysplasia (SMC). DMC is a rare autosomal recessive disorder characterized by short trunk dwarfism, microcephaly and psychomotor retardation, with cutaneous cells containing dilated rough endoplasmic reticulum, enlarged and aberrant vacuoles and numerous vesicles [PubMed: 12491225]. Distinct with features of DMS, SMC is a rare osteochondrodysplasia characterized by short limbs and trunk with barrel-shaped chest [PubMed: 19005420]. Dymeclin is a not a stably anchored transmembrane protein with a transmembrane domain, but it shuttles between Golgi and cytosol, which is necessary for correct organization of Golgi apparatus [PubMed: 18996921].
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Dymeclin antibody 12001-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Dymeclin Polyclonal antibody (Cat# 12001-1-AP) has 1 citation. It appears in Molecular Medicine (IF 8.3), published in 2026. The cited study investigates Dymeclin's role in chronic unpredictable mild stress-induced depression, focusing on maintaining Golgi apparatus structure and regulating NLRP3 inflammasome activation. The research fields encompass neuroscience (depression modeling) and cell biology (Golgi organization and inflammasome signaling).
| Species | Application | Title |
|---|---|---|
Mol Med The role of Dymeclin in chronic unpredictable mild stress-induced depression: maintaining the Golgi apparatus structure and regulating NLRP3 inflammasome activation. |
