DYNAP Polyclonal antibody

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• DYNAP Polyclonal antibody (32123-1-AP)

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  WB analysis using 32123-1-AP

  U2OS cells lysate was subjected to SDS PAGE followed by western blot with 32123-1-AP (DYNAP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

DYNAP Polyclonal Antibody for WB, ELISA

Cat No. 32123-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

C18orf26, Dynactin-associated protein, Full

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Tested Applications

• Positive WB detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32123-1-AP targets DYNAP in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag37125

  Product name: Recombinant human C18orf26 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 135-210 aa of NM_001307955

  Sequence: LVNNKGSANSSIVIQLSTNDGECVTVKPGTPSPACPPTMTTTSTVPASTATESTTSTATAATTSTEPITVAPTDHL

• Full Name: chromosome 18 open reading frame 26
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: NM_001307955
• Gene Symbol: C18orf26
• Gene ID (NCBI): 284254
• RRID: AB_3742530
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q8N1N2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The dynactin-associated protein (DYNAP), also known as C18orf26, is a transmembrane protein that localises to the Golgi apparatus and the plasma membrane in a microtubule-dependent manner. This protein is expressed in half of all human cancer cell lines. DYNAP expression activates Akt (also known as protein kinase B) by promoting Ser473 phosphorylation, which is required for full activation (PMID: 20978158). In SDS-PAGE, DYNAP migrates anomalously at ~45 kDa, which is much larger than the expected 22.5 kDa based on its amino acid composition. The T/S cluster region is responsible for this (PMID: 35022995).