DYNC1H1 Recombinant antibody

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  WB analysis using 85397-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85397-4-RR (KIAA0325 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

DYNC1H1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85397-4-RR

Clone No.242706B12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

DYNC1H 1

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, HEK-293T cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85397-4-RR targets DYNC1H1 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DYNC1H1 fusion protein Ag2999
• Full Name: dynein, cytoplasmic 1, heavy chain 1
• Calculated Molecular Weight: 4646 aa, 532 kDa
• Observed Molecular Weight: 532 kDa
• GenBank Accession Number: BC021297
• Gene Symbol: DYNC1H1
• Gene ID (NCBI): 1778
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q14204
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dyneins are a group of microtubule-activated ATPases that convert chemical energy into mechanical energy. It can be divided into 2 large subgroups, namely, the axonemal and cytoplasmic dyneins. The conventional cytoplasmic dynein comprises 2 heavy chain polypeptides and several intermediate and light chains. DYNC1H1 is a cytoplasmic dynein and belongs to the dynein heavy chain family. It acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. DYNC1H1 has been implicated in the degeneration of dopaminergic neuron axons and motor neurons in PD patients.