Tested Applications
| Positive WB detected in | U-87 MG cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HUVEC cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13808-1-AP targets DYNC1I1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4810 Product name: Recombinant human DYNC1I1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 276-625 aa of BC022540 Sequence: SLQYPELMVASYNNNEDAPHEPDGVALVWNMKFKKTTPEYVFHCQSSVMSVCFARFHPNLVVGGTYSGQIVLWDNRSHRRTPVQRTPLSAAAHTHPVYCVNVVGTQNAHNLITVSTDGKMCSWSLDMLSTPQESMELVYNKSKPVAVTGMAFPTGDVNNFVVGSEEGTVYTACRHGSKAGIGEVFEGHQGPVTGINCHMAVGPIDFSHLFVTSSFDWTVKLCTTKHNKPLYSFEDNADYVYDVMWSPVHPALFACVDGMGRLDLWNLNNDTEVPTASVAIEGASALNRVRWAQAGKEVAVGDSEGRIWVYDVGELAVPHNDEWTRFARTLVEIRANRADSEEEGTVELSA Predict reactive species |
| Full Name | dynein, cytoplasmic 1, intermediate chain 1 |
| Calculated Molecular Weight | 645 aa, 71 kDa |
| Observed Molecular Weight | 71 kDa |
| GenBank Accession Number | BC022540 |
| Gene Symbol | DYNC1I1 |
| Gene ID (NCBI) | 1780 |
| RRID | AB_2093492 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14576 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DYNC1I1 antibody 13808-1-AP | Download protocol |
| IHC protocol for DYNC1I1 antibody 13808-1-AP | Download protocol |
| WB protocol for DYNC1I1 antibody 13808-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DYNC1I1 Polyclonal antibody (Cat# 13808-1-AP) has 6 citations published in Oncogene, EMBO Journal, Neurobiology of Disease, Experimental Gerontology, iScience, and Frontiers in Molecular Neuroscience. Research fields span neurodegeneration (Alzheimer's disease), cancer (glioblastoma, rhabdomyosarcoma), mitochondrial transport and dynamics, pain sensitivity, and axonal trafficking in neurons.
| Species | Application | Title |
|---|---|---|
Oncogene Cytoplasmic dynein regulates the subcellular localization of sphingosine kinase 2 to elicit tumor-suppressive functions in glioblastoma.
| ||
EMBO J Annexin A7 enhances TIA1 axonal trafficking to counteract pathological aggregation in neurons. | ||
Neurobiol Dis Pain sensitivity related to gamma oscillation of parvalbumin interneuron in primary somatosensory cortex in Dync1i1-/- mice
| ||
Exp Gerontol The potential benefits of PGC-1α in treating Alzheimer's disease are dependent on the integrity of the LLKYL L3 motif: Effect of regulating mitochondrial axonal transportation | ||
iScience High level dynein impairs mitochondrial distribution and differentiation of rhabdomyosarcoma cells. | ||
Front Mol Neurosci MFN2 Deficiency Impairs Mitochondrial Transport and Downregulates Motor Protein Expression in Human Spinal Motor Neurons |
Reviews
What customers say
One reviewer (Q. Chen) rated this antibody 4/5 stars for Western Blot on mouse primary neurons at a 1:500 dilution. They described it as an OK antibody for detecting DIC1, noting that the signal is somewhat weak and requires strong ECL for detection. Overall, the feedback is positive but suggests users should expect to optimize exposure conditions for best results.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Q (Verified Customer) (03-22-2021) | An OK antibody to detect DIC1, although the signal is a little weak and strong ECL is required.
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