CoraLite®594-conjugated DYNC2H1 Polyclonal antibody

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  IF Staining of hTERT-RPE1 using CL594-29758

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite®594 DYNC2H1 antibody (CL594-29758) at dilution of 1:200, CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

DYNC2H1 Polyclonal Antibody for IF/ICC

Cat No. CL594-29758

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

Synonyms

DHC1b, DHC2, DNCH2, DYH1B, hdhc11

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Tested Applications

• Positive IF/ICC detected in: hTERT-RPE1 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-29758 targets DYNC2H1 in applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DYNC2H1 fusion protein Ag30707
• Full Name: dynein, cytoplasmic 2, heavy chain 1
• Calculated Molecular Weight: 493 kDa
• Observed Molecular Weight: 493 kDa
• GenBank Accession Number: NM_001377
• Gene Symbol: DYNC2H1
• Gene ID (NCBI): 79659
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NCM8
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DYNC2H1 (Cytoplasmic dynein 2 heavy chain 1) is involved in ciliary intraflagellar transport (IFT). DYNC2H1 drives retrograde transport of the IFT-A protein complex that regulates tip-to-base transport in cilia, involved in the generation and maintenance of cilia. DYNC2H1 variants or retina-predominant variants cause nonsyndromic retinal degeneration (PMID:32753734). DYNC2H1 mutations also cause asphyxiating thoracic dystrophy and short rib-polydactyly syndrome (PMID: 19442771). DYNC2H1 was localized to the basal bodies by immunofluorescence staining (PMID: 21552265, PMID: 26077881, PMID: 30320547).