Tested Applications
| Positive WB detected in | A431 cells, human heart tissue, mouse brain tissue, rat brain tisssue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16811-1-AP targets DYNLL2 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10345 Product name: Recombinant human DYNLL2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-89 aa of BC010744 Sequence: MSDRKAVIKNADMSEDMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG Predict reactive species |
| Full Name | dynein, light chain, LC8-type 2 |
| Calculated Molecular Weight | 89 aa, 10 kDa |
| Observed Molecular Weight | 10 kDa |
| GenBank Accession Number | BC010744 |
| Gene Symbol | DYNLL2 |
| Gene ID (NCBI) | 140735 |
| RRID | AB_2093667 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96FJ2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DYNLL2 antibody 16811-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DYNLL2 polyclonal antibody (Cat# 16811-1-AP) has 14 citations spanning journals including Science, Current Biology, Cell Reports, eLife, Development, Analytical Chemistry, Cell Cycle, Frontiers in Oncology, Asian Journal of Andrology, Biochemical Pharmacology, Cellular and Molecular Life Sciences, Scientific Reports, Biochimica et Biophysica Acta, and International Journal of Biological Sciences. Research fields include cilia/flagella biogenesis, sperm motility and male infertility, microtubule dynamics, protein-protein interactions, pyroptosis in sepsis, and osteosarcoma biomarkers.
| Species | Application | Title |
|---|---|---|
Int J Biol Sci Identification and Functional Characterization of a New Splicing Variant of EZH2 in the Central Nervous System. | ||
Curr Biol CCDC176 stabilizes microtubule doublets 1 and 9 to ensure proper sperm movement | ||
Cell Rep Differential requirements of IQUB for the assembly of radial spoke 1 and the motility of mouse cilia and flagella | ||
Anal Chem Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes. | ||
Cell Mol Life Sci CCDC146 is required for sperm flagellum biogenesis and male fertility in mice | ||
Biochem Pharmacol Targeting the DYNLL2-PAK1 axis inhibits caspase-11-dependent pyroptosis to alleviate sepsis.
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