DYRK1A Polyclonal antibody

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  WB analysis using 28607-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28607-1-AP (DYRK1A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

DYRK1A Polyclonal Antibody for WB, ELISA

Cat No. 28607-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

DYRK, DYRK1, hMNB, HP86, MNB

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Tested Applications

• Positive WB detected in: DU 145 cells, HEK-293 cells, LNCaP cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28607-1-AP targets DYRK1A in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag29433

  Product name: Recombinant human DYRK1A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 525-763 aa of NM_001396

  Sequence: GRARSDPTHQHRHSGGHFTAAVQAMDCETHSPQVRQQFPAPLGWSGTEAPTQVTVETHPVQETTFHVAPQQNALHHHHGNSSHHHHHHHHHHHHHGQQALGNRTRPRVYNSPTNSSSTQDSMEVGHSHHSMTSLSSSTTSSSTSSSSTGNQGNQAYQNRPVAANTLDFGQNGAMDVNLTVYSNPRQETGIAGHPTYQFSANTGPAHYMTEGHLTMRQGADREESPMTGVCVQQSPVASS

• Full Name: dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A
• Calculated Molecular Weight: 86 kDa
• Observed Molecular Weight: 70kDa, 85 kDa
• GenBank Accession Number: NM_001396
• Gene Symbol: DYRK1A
• Gene ID (NCBI): 1859
• RRID: AB_3086069
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13627
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This gene encodes a member of the Dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. This member contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. It catalyzes its autophosphorylation on serine/threonine and tyrosine residues. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. This gene is a homolog of Drosophila mnb (minibrain) gene and rat Dyrk gene. It is localized in the Down syndrome critical region of chromosome 21, and is considered to be a strong candidate gene for learning defects associated with Down syndrome.