DYRK1B Polyclonal antibody

DYRK1B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12240-1-AP

Synonyms

DYRK1B, Minibrain related kinase, MIRK, Mirk protein kinase

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Validation Data Gallery

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  WB analysis of mouse testis using 12240-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12240-1-AP (DYRK1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 12240-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12240-1-AP (DYRK1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 12240-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 12240-1-AP (DYRK1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 12240-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12240-1-AP (DYRK1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12240-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12240-1-AP (DYRK1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse testis tissue, HeLa cells, human colon tissue, human heart tissue, mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12240-1-AP targets DYRK1B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DYRK1B fusion protein Ag2883
• Full Name: dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B
• Calculated Molecular Weight: 629 aa, 69 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC018751
• Gene Symbol: DYRK1B
• Gene ID (NCBI): 9149
• RRID: AB_2093803
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DYRK1B(dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B) is also named as MIRK. It is a member of the dyrk/minibrain family of serine/threonine kinases that mediate the transition from growth to differentiation in lower eukaryotes and mammals(PMID:15546868). DYRK1B functions as a transcriptional coactivator that is activated by co-expressed MKK3, a MAP kinase that also can activate p38 MAP kinase(PMID:14500717). The DYRK1B splice variants found in each muscle tissue exhibit approximate molecular weights of 70 and 75 kDa(PMID:12902328). It has 3 isoforms produced by alternative splicing of the molecular weight of 65-69 kDa.

Publications

Species	Application	Title
human	WB	Am J Physiol Cell Physiol; MicroRNA-9 enhances the transactivation of nuclear factor of activated T cells by targeting KPNB1 and DYRK1B.; Authors - Yan Zeng