DYX1C1 Polyclonal antibody

• Featured Product
• KD/KO Validated

DYX1C1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 14522-1-AP

Synonyms

DYX1, DYX1C1, DYXC1, EKN1, RD

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Validation Data Gallery

• 

  WB analysis using 14522-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 14522-1-AP (DYX1C1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 14522-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14522-1-AP (DYX1C1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 14522-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 14522-1-AP (DYX1C1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 14522-1-AP

  IP result of anti-DYX1C1 (IP:14522-1-AP, 4ug; Detection:14522-1-AP 1:800) with mouse testis tissue lysate 1120 ug.

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Tested Applications

• Positive WB detected in: rat testis tissue, mouse testis tissue
• Positive IP detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

14522-1-AP targets DYX1C1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DYX1C1 fusion protein Ag5997
• Full Name: dyslexia susceptibility 1 candidate 1
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC062564
• Gene Symbol: DYX1C1
• Gene ID (NCBI): 161582
• RRID: AB_2093955
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DYX1C1 initially was described as the first candidate gene for developmental dyslexia. The protein contains three TPR motifs and one p23 domain, all commonly known for their protein-protein interactions. It also has been linked to neuronal migration. Recently it has been reported that the expression of DYX1C1 protein is lost in breast cancer and patients who have lost DYX1C1 expression have a poor overall survival,indicating that DYX1C1 is a potential prognostic biomarker in breast cancer.

Publications

Species	Application	Title
mouse	WB	Nat Genet; DYX1C1 is required for axonemal dynein assembly and ciliary motility.; Authors - Tarkar Aarti A ; KO Validated
human	IF	Biol Psychiatry; Molecular networks of DYX1C1 gene show connection to neuronal migration genes and cytoskeletal proteins.; Authors - Kristiina Tammimies
human	WB,IF	Mol Endocrinol; The rs3743205 SNP is important for the regulation of the dyslexia candidate gene DYX1C1 by estrogen receptor β and DNA methylation.; Authors - Tammimies Kristiina K
human	IHC	BMC Cancer; The dyslexia candidate gene DYX1C1 is a potential marker of poor survival in breast cancer.; Authors - Rosin Gustaf G
human	WB,IP	BMC Mol Cell Biol; Genetic and protein interaction studies between the ciliary dyslexia candidate genes DYX1C1 and DCDC2; Authors - Andrea Bieder