DZIP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

DZIP1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 13779-1-AP

Synonyms

DAZ interacting protein 1, DAZ interacting protein 1/2, DZIP, DZIP1, DZIP2, DZIPt1, KIAA0996, RP11 23E3.3, Zinc finger protein DZIP1

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Validation Data Gallery

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  WB analysis of HEK-293 using 13779-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13779-1-AP (DZIP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 13779-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 13779-1-AP (DZIP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 13779-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13779-1-AP (DZIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13779-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13779-1-AP (DZIP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13779-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13779-1-AP (DZIP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 13779-1-AP

  IP result of anti-DZIP1 (IP:13779-1-AP, 4ug; Detection:13779-1-AP 1:300) with HEK-293 cells lysate 880ug.

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  IF Staining of PC-3 using 13779-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 13779-1-AP (DZIP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, human brain tissue, human testis tissue, mouse skeletal muscle tissue, PC-3 cells
• Positive IP detected in: HEK-293 cells
• Positive IF detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 3 publications below

Product Information

13779-1-AP targets DZIP1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DZIP1 fusion protein Ag4772
• Full Name: DAZ interacting protein 1
• Calculated Molecular Weight: 867 aa, 98 kDa
• Observed Molecular Weight: 100-113 kDa
• GenBank Accession Number: BC041804
• Gene Symbol: DZIP1
• Gene ID (NCBI): 22873
• RRID: AB_2096927
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DZIP1, also named as DZIP, DZIP2 and KIAA0996, belongs to the DZIP C2H2-type zinc-finger protein family. DZIP1 has been reported to be associated with centrosomes and ciliogenesis in cells (PMID: 32051257). DZIP1 may participate in spermatogenesis via its interaction with DAZ1, and play a role in primary formation (PMID: 15081113, 19852954). DZIP1 has 3 isoforms with the molecular mass of 31, 97 and 99 kDa. It may be a potential therapeutic target of oral squamous carcinoma (PMID: 31450126).

Publications

Species	Application	Title
mouse	IF	Nat Genet; Mutations in DZIP1L, which encodes a ciliary-transition-zone protein, cause autosomal recessive polycystic kidney disease.; Authors - Hao Lu
human	IF	Dev Biol; A function for the Joubert syndrome protein Arl13b in ciliary membrane extension and ciliary length regulation.; Authors - Hao Lu
mouse,human	WB,IF	Dev Dyn; DZIP1 regulates mammalian cardiac valve development through a Cby1-β-catenin mechanism.; Authors - Lilong Guo ; KO Validated