Mouse DFNA5/GSDME Recombinant monoclonal antibody, PBS Only (Detector)

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  WB analysis using 82955-2-RR (same clone as 82955-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82955-2-RR (Dfna5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82955-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP02686-1

  The mean DFNA5/GSDME concentration was determined to be 85.78 ng/mL in J744A.1 cell extract based on a 1.3 mg/mL extract load, 16.86 ng/mL in mouse brain tissue extract based on a 7.0 mg/mL extract load and 10.69 ng/mL in rat brain tissue extract based on a 8.2 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02686-1

  Sandwich ELISA standard curve of MP02686-1, Mouse DFNA5/GSDME Recombinant Matched Antibody Pair - PBS only. 82955-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag33622. 82955-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL

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  Affinity and Kinetic Characterization of 82955-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82955-2-RR against Mouse DFNA5/GSDME were performed. The affinity constant is below 1 pM.

DFNA5/GSDME Uni-rAb^® Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA

Cat No. 82955-2-PBS

Clone No.251415B1

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, Sandwich ELISA, Indirect ELISA

Synonyms

Dfna5, Dfna5h, EG14210, Fin15, Gasdermin-E

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Product Information

82955-2-PBS targets DFNA5/GSDME as part of a matched antibody pair:

MP02686-1: 82955-3-PBS capture and 82955-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag33622

  Product name: Recombinant mouse Dfna5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-200 aa of BC132303

  Sequence: MFAKATRNFLKEVDAGGDLISVSHLNDSDKLQLLSLVTKKKRYWCWQRPKYQILSATLEDVLTEGHCLSPVVVESDFVKYESKCENHKSGAIGTVVGKVKLNVGGKGVVESHSSFGTLRKQEVDVQQLIQDAVKRTVNMDNLVLQQVLESRNEVLCVLTQKIMTTQKCVISEHVQSEETCGGMVGIQTKTIQVSATEDGT*

• Full Name: deafness, autosomal dominant 5 (human)
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC132303
• Gene Symbol: DFNA5/GSDME
• Gene ID (NCBI): 54722
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Z2D3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain.