Di-Methyl-Histone H3 (Lys9) Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 86990-2-RR (same clone as 86990-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86990-2-RR (Di-Methyl-Histone H3 (Lys9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86990-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 86990-2-RR (same clone as 86990-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Di-Methyl-Histone H3 (Lys9) antibody (86990-2-RR, Clone: 252046G7 ) at dilution of 1:2500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86990-2-PBS in a different storage buffer formulation.

• 

  Dot Blot experiment of / using 86990-2-RR (same clone as 86990-2-PBS)

  Dot blot analysis was used to confirm the specificity of 86990-2-RR Di-Methyl-Histone H3 (Lys9) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 86990-2-PBS in a different storage buffer formulation.

• 

  ChIP experiment of HeLa using 86990-2-RR (same clone as 86990-2-PBS)

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Di-Methyl-Histone H3 (Lys9) (86990-2-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 86990-2-PBS in a different storage buffer formulation.

Di-Methyl-Histone H3 (Lys9) Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR

Cat No. 86990-2-PBS

Clone No.252046G7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR

Synonyms

Di Methyl Histone H3, Di-Methyl Histone H3 (Lys9), Di-Methyl Histone H3 Lys9, Di-Methyl Histone-H3 Lys9, Di-Methyl-Histone H3

---

Product Information

86990-2-PBS targets Di-Methyl-Histone H3 (Lys9) in WB, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: histone cluster 1, H3a
• Observed Molecular Weight: 15-17 kDa
• GenBank Accession Number: BC066245
• Gene Symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P68431
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

H3K9me2 is a typical transcriptional repressive modification, primarily enriched in heterochromatin regions (such as centromeres, telomeres, and transposon elements), but can also be scattered throughout euchromatin. By recruiting "reader" proteins such as HP1 (Heterochromatin Protein 1), H3K9me2 can establish and stabilize a chromatin condensation state, thereby silencing gene expression.