Various lysates were subjected to SDS PAGE followed by western blot with 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 84908-2-RR
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 84908-2-RR
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 84908-2-RR
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 84908-2-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Di/Tri-Methyl-Histone H3 (Lys4) antibody (84908-2-RR, Clone: 241942D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Di/Tri-Methyl-Histone H3 (Lys4) antibody (84908-2-RR, Clone: 241942D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
Dot Blot experiment of peptide using 84908-2-RR
Dot blot analysis was used to confirm the specificity of 84908-2-RR Di/Tri-Methyl-Histone H3 (Lys4) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.
Dot blot analysis was used to confirm the specificity of 84908-2-RR Di/Tri-Methyl-Histone H3 (Lys4) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.
ChIP experiment of HeLa using 84908-2-RR
Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Di/Tri-Methyl-Histone H3 (Lys4) (84908-2-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.
Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Di/Tri-Methyl-Histone H3 (Lys4) (84908-2-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.
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mouse testis tissue, mouse liver tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Positive Dot Blot detected in
peptide
Positive ChIP-qPCR detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:2000-1:10000
Immunohistochemistry (IHC)
IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC
IF/ICC : 1:500-1:2000
DOT BLOT
DOT BLOT : 1:10-1:100
CHIP-QPCR
CHIP-QPCR : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Various lysates were subjected to SDS PAGE followed by western blot with 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse testis using 84908-2-RR
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 84908-2-RR
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 84908-2-RR
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 84908-2-RR
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 84908-2-RR
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 84908-2-RR
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of HeLa using 84908-2-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Di/Tri-Methyl-Histone H3 (Lys4) antibody (84908-2-RR, Clone: 241942D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
DOT BLOT Figures
Dot Blot experiment of peptide using 84908-2-RR
Dot blot analysis was used to confirm the specificity of 84908-2-RR Di/Tri-Methyl-Histone H3 (Lys4) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.
CHIP-QPCR Figures
ChIP experiment of HeLa using 84908-2-RR
Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Di/Tri-Methyl-Histone H3 (Lys4) (84908-2-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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