ChromoTek Dnmt1-Chromobody® plasmid (TagGFP2)

DNA plasmid encoding for anti-Dnmt1 VHH (anti-Dnmt1 Nanobody) fused to TagGFP2.

Vector type

Mammalian expression vector

Promoter

Constitutive CMV IE

Reporter

TagGFP2

Epitope

Dmnt1

Cat no : dcg

Synonyms



Product Information

DNA plasmid encoding for anti-Dnmt1 VHH (anti-Dnmt1 Nanobody) fused to TagGFP2.

Applications Live cell imaging
SpecificityDnmt1 (DNA (cytosine-5)-methyltransferase 1), tested in human cells
Vector typeMammalian expression vector
PromoterConstitutive CMV IE
ReporterTagGFP2
Codon usageMammalian
SelectionKan/Neo
Encoded Nanobody/ VHHMonoclonal anti-Dnmt1 single domain antibody (sdAb) fragment
SequenceWith the Cell Cycle-Chromobody plasmid you receive the sequence information of the Alpaca antibody to PCNA fused to TagRFP, as well as the full vector sequence.
Microscopy techniquesWide-field epifluorescence microscopy; confocal microscopy; super-resolution microscopy e.g. STED and 3D-SIM; high-content microscopy and analysis
TransfectionTransfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
StorageShipped at ambient temperature. Store at -20 °C