ChromoTek Dnmt1-Chromobody® plasmid (TagRFP)
DNA plasmid encoding for anti-Dnmt1 VHH (anti-Dnmt1 Nanobody) fused to TagRFP.
Vector type
Mammalian expression vector
Promoter
Constitutive CMV IE
Reporter
TagRFP
Epitope
Dmnt1
Cat no : dcr
Synonyms
Validation Data Gallery
Product Information
DNA plasmid encoding for anti-Dnmt1 VHH (anti-Dnmt1 Nanobody) fused to TagRFP.
Applications | Live cell imaging |
Specificity | Dnmt1 (DNA (cytosine-5)-methyltransferase 1), tested in human cells |
Vector type | Mammalian expression vector |
Promoter | Constitutive CMV IE |
Reporter | TagRFP |
Codon usage | Mammalian |
Selection | Kan/Neo |
Encoded Nanobody/ VHH | Monoclonal anti-Dnmt1 single domain antibody (sdAb) fragment |
Sequence | With the Cell Cycle-Chromobody plasmid you receive the sequence information of the Alpaca antibody to PCNA fused to TagRFP, as well as the full vector sequence. |
Microscopy techniques | Wide-field epifluorescence microscopy; confocal microscopy; super-resolution microscopy e.g. STED and 3D-SIM; high-content microscopy and analysis |
Transfection | Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type. |
Storage Condition | Shipped at ambient temperature. Store at -20 °C |
Documentation
SDS |
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SDS Dnmt1-Chromobody® TagGFP (PDF) |
Datasheet |
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Datasheet Dnmt1-Chromobody® TagRFP (PDF) |
Datasheet Dnmt1-Chromobody® TagGFP (PDF) |
Brochure |
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Chromotek nanobodies brochure (PDF) |
EULA |
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End User License Agreement EULA Dnmt1 Chromobody TagGFP plasmid non-profit (PDF) |
End User License Agreement EULA Dnmt1 Chromobody TagRFP plasmid non-profit (PDF) |
Publications
Application | Title |
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