Validation Data Gallery
Product Information
DNA plasmid encoding for anti-Dnmt1 VHH (anti-Dnmt1 Nanobody) fused to TagRFP.
| Description | The Dnmt1-Chromobody visualizes endogenous Dnmt1 in real-time during cell cycle progression. • Trace dynamic changes during the cell cycle in real time • Monitor the distribution of an endogenous cell cycle marker protein without overexpression artifacts or cytotoxic effects from overexpressed fluorescent protein fusion Dnmt1 |
| Applications | Live cell imaging |
| Specificity | Dnmt1 (DNA (cytosine-5)-methyltransferase 1), tested in human cells |
| Reporter | TagRFP |
| Vector type | Mammalian expression vector |
| Promoter | Constitutive CMV IE |
| Codon usage | Mammalian |
| Selection | Kan/Neo |
| Sequence | With the Cell Cycle-Chromobody plasmid you receive the sequence information of the Alpaca antibody to PCNA fused to TagRFP, as well as the full vector sequence. |
| Transfection | Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type. |
| Microscopy techniques | Wide-field epifluorescence microscopy; confocal microscopy; super-resolution microscopy e.g. STED and 3D-SIM; high-content microscopy and analysis |
| Storage Condition | Shipped at ambient temperature. Store at -20 °C |
Documentation
| SDS |
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| dcr_SDS_Dnmt1-Chromobody® plasmid (TagRFP) (EN) |
| Datasheet |
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| Datasheet Dnmt1-Chromobody® TagRFP (PDF) |
| EULA |
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| dcr End User License Agreement (EULA) Dnmt1 Chromobody TagRFP plasmid non-profit (PDF) |
| dcr End User License Agreement (EULA) Dnmt1 Chromobody TagRFP plasmid non-profit (US) (PDF) |
| Brochure |
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| Chromotek nanobodies brochure (PDF) |
Publications
| Application | Title |
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