E-Cadherin Recombinant antibody

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  WB analysis using 84646-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84646-3-RR (E-Cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat colon using 84646-3-RR

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 84646-3-RR (E-Cadherin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat colon using 84646-3-RR

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 84646-3-RR (E-Cadherin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84646-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84646-3-RR against Rat E-Cadherin were performed. The affinity constant is 1.84 nM.

E-Cadherin Recombinant Antibody for WB, IHC, ELISA

Cat No. 84646-3-RR

Clone No.242018D3

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Synonyms

242018D3

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Tested Applications

• Positive WB detected in: Caco-2 cells, A431 cells
• Positive IHC detected in: rat colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84646-3-RR targets E-Cadherin in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: cadherin 1
• Calculated Molecular Weight: 99 kDa
• Observed Molecular Weight: 125 kDa
• GenBank Accession Number: NM_031334.1
• Gene Symbol: Cdh1
• Gene ID (NCBI): 83502
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9R0T4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.