E-cadherin Recombinant monoclonal antibody, PBS Only

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• E-cadherin Recombinant monoclonal antibody, PBS Only (83991-4-PBS)

E-cadherin Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 83991-4-PBS Copied!

Clone No. 241098G6

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

241098G6, Arc 1, Cadherin 1, Cadherin-1, CAM 120/80

• 

  WB analysis using 83991-4-RR (same clone as 83991-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83991-4-RR (E-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 83991-4-RR (same clone as 83991-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83991-4-RR (E-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 83991-4-RR (same clone as 83991-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 83991-4-RR (same clone as 83991-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 83991-4-RR (same clone as 83991-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 83991-4-RR (same clone as 83991-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary cancer using 83991-4-RR (same clone as 83991-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary cancer using 83991-4-RR (same clone as 83991-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 83991-4-RR (same clone as 83991-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 83991-4-RR (same clone as 83991-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colorectal cancer using 83991-4-RR (same clone as 83991-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 83991-4-RR (same clone as 83991-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 83991-4-RR (same clone as 83991-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary cancer using 83991-4-RR (same clone as 83991-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 83991-4-RR (same clone as 83991-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 83991-4-RR (same clone as 83991-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human appendicitis using 83991-4-RR (same clone as 83991-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 83991-4-RR (same clone as 83991-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A431 using 83991-4-RR (same clone as 83991-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using E-cadherin antibody (83991-4-RR, Clone: 241098G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of MCF-7 using 83991-4-RR (same clone as 83991-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using E-cadherin antibody (83991-4-RR, Clone: 241098G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 83991-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83991-4-RR against Human E-cadherin were performed. The affinity constant is 2.78 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

83991-4-PBS targets E-cadherin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1112

  Product name: Recombinant Human E-cadherin protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 155-709 aa of NM_004360.5

  Sequence: DWVIPPISCPENEKGPFPKNLVQIKSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSNGNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNTYNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRESFPTYTLVVQAADLQGEGLSTTATAVITVTDTNDNPPIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPNTSPFTAELTHGASANWTIQYNDPTQESIILKPKMALEVGDYKINLKLMDNQNKDQVTTLEVSVCDCEGAAGVCRKAQPVEAGLQIPA

• Full Name: cadherin 1, type 1, E-cadherin (epithelial)
• Calculated Molecular Weight: 97 kDa
• Observed Molecular Weight: 135 kDa
• GenBank Accession Number: NM_004360.5
• Gene Symbol: E-cadherin
• Gene ID (NCBI): 999
• RRID: AB_3671566
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P12830-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.