Anti-Human E-cadherin Rabbit Recombinant Antibody

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  FC experiment of MCF-7 using 98030-1-RR

  1x10^6 MCF-7 cells (left) or Jurkat cells (right) were surface stained with 0.25 ug Anti-Human CD324 (E-cadherin) Rabbit Recombinant Antibody (98030-1-RR, Clone: 240159G8) (red) or Isotype Control (blue), and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  FC experiment of HT-29 using 98030-1-RR

  1x10^6 HT-29 cells (left) or Jurkat cells (right) were surface stained with 0.25 ug Anti-Human CD324 (E-cadherin) Rabbit Recombinant Antibody (98030-1-RR, Clone:240159G8) (red) or Isotype Control (blue), and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98030-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98030-1-RR against Human E-cadherin were performed. The affinity constant is 37.3 pM.

E-cadherin Uni-rAb^® Recombinant Antibody for FC

Cat No. 98030-1-RR

Clone No.240159G8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD324 (E-cadherin), 240159G8, Arc 1, Cadherin 1, Cadherin-1

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Tested Applications

• Positive FC detected in: MCF-7 cells, HT-29 cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98030-1-RR targets E-cadherin in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag14973

  Product name: Recombinant human E-cadherin protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 373-622 aa of BC141838

  Sequence: PIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPI

• Full Name: cadherin 1, type 1, E-cadherin (epithelial)
• Calculated Molecular Weight: 882 aa, 97 kDa
• GenBank Accession Number: BC141838
• Gene Symbol: E-cadherin
• Gene ID (NCBI): 999
• RRID: AB_3672175
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P12830
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CD324, CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.