Multi-rAb® E-cadherin Multi-Recombinant antibody

• Home
• Products
• Primary Antibodies
• Multi-rAb® E-cadherin Multi-Recombinant antibody (RMX00039)

E-cadherin Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. RMX00039

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Arc 1, Cadherin 1, Cadherin-1, CAM 120/80, CD324

• 

  WB analysis using RMX00039

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00039 (E-cadherin antibody) at dilution of 1:70000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using RMX00039

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using RMX00039 (E-cadherin antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using RMX00039

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using RMX00039 (E-cadherin antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of MCF-7 using RMX00039

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using E-cadherin antibody (RMX00039) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

---

Tested Applications

• Positive WB detected in: A431 cells, MCF-7 cells, T-47D cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochemistry (IHC): IHC : 1:4000-1:16000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00039 targets E-cadherin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: cadherin 1, type 1, E-cadherin (epithelial)
• Calculated Molecular Weight: 882 aa, 97 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC141838
• Gene Symbol: E-cadherin
• Gene ID (NCBI): 999
• RRID: AB_3750092
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: P12830
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness. E-cadherin is sensitive to trypsin digestion in the absence of Ca2+. This polyclonal antibody recognizes 120-125 kDa intact E-cadherin and its cleaved fragments of 80-120 kDa.