Anti-Mouse E-selectin/CD62E Rabbit Recombinant Antibody, PBS Only

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  FC experiment of bEnd.3 using 98065-1-RR (same clone as 98065-1-PBS)

  1x10^6 bEnd.3 cells were surface stained with 0.25 ug Anti-Mouse E-selectin/CD62E Rabbit Recombinant Antibody (98065-1-RR, Clone:240700D4) (red) or isotype control (blue) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG. Cells were not fixed. This data was developed using the same antibody clone with 98065-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98065-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98065-1-RR against Mouse E-selectin/CD62E were performed. The affinity constant is 4.40 nM.

E-selectin/CD62E Uni-rAb^® Recombinant Antibody for FC

Cat No. 98065-1-PBS

Clone No.240700D4

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

CD62E, E-selectin, 240700D4, E selectin, Elam

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Product Information

98065-1-PBS targets E-selectin/CD62E in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: selectin, endothelial cell
• Calculated Molecular Weight: 67KD
• GenBank Accession Number: NM_011345.2
• Gene Symbol: Sele
• Gene ID (NCBI): 20339
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q00690
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

E-selectin, also known as CD62E or ELAM-1, is a member of the selectin family of adhesion molecules that also includes L-selectin and P-selectin. E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS). Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. (PMID: 2827173; 1382417; 8943958)