E2F1 Recombinant monoclonal antibody

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  WB analysis using 87313-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87313-3-RR (E2F1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 87313-3-RR

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 87313-3-RR (E2F1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

E2F1 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87313-3-RR

Clone No.252660D5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

E2F 1, E2F-1, pRB-binding protein E2F-1, RBAP-1, RBBP-3

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Tested Applications

• Positive WB detected in: Jurkat cells, NIH/3T3 cells, LNCap cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87313-3-RR targets E2F1 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag17363

  Product name: Recombinant human E2F1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 88-438 aa of BC050369

  Sequence: VKRRLDLETDHQYLAESSGPARGRGRHPGKGVKSPGEKSRYETSLNLTTKRFLELLSHSADGVVDLNWAAEVLKVQKRRIYDITNVLEGIQLIAKKSKNHIQWLGSHTTVGVGGRLEGLTQDLRQLQESEQQLDHLMNICTTQLRLLSEDTDSQRLAYVTCQDLRSIADPAEQMVMVIKAPPETQLQAVDSSENFQISLKSKQGPIDVFLCPEETVGGISPGKTPSQEVTSEEENRATDSATIVSPPPSSPPSSLTTDPSQSLLSLEQEPLLSRMGSLRAPVDEDRLSPLVAADSLLEHVREDFSGLLPEEFISLSPPHEALDYHFGLEEGEGIRDLFDCDFGDLTPLDF

• Full Name: E2F transcription factor 1
• Calculated Molecular Weight: 437 aa, 47 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC050369
• Gene Symbol: E2F1
• Gene ID (NCBI): 1869
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q01094
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transcription factor E2F1 (E2F1), also known as RBBP3, is a transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. The sumoylated E2F1 is bout 55-60 kDa.