E2F4 Monoclonal antibody

E2F4 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

2D1E1

Cat no : 67812-1-Ig

Synonyms

E2F 4, E2F4, Transcription factor E2F4



Tested Applications

Positive WB detected inLNCaP cells, HSC-T6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

67812-1-Ig targets E2F4 in WB,ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen E2F4 fusion protein Ag28533
Full Name E2F transcription factor 4, p107/p130-binding
Calculated molecular weight 44 kDa
GenBank accession numberBC033180
Gene symbol E2F4
Gene ID (NCBI) 1874
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Transcription factor E2F4 (E2F4) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.The observed molecular weight of 60-70 kDa is the phosphorylation of E2F4 protein.

Protocols

Product Specific Protocols
WB protocol for E2F4 antibody 67812-1-IgDownload protocol
Standard Protocols
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