E2F4 Monoclonal antibody

E2F4 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1


Human, mouse, rat







Cat no : 67812-1-Ig


E2F 4, E2F4, Transcription factor E2F4

Tested Applications

Positive WB detected inLNCaP cells, HSC-T6 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67812-1-Ig targets E2F4 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen E2F4 fusion protein Ag28533
Full Name E2F transcription factor 4, p107/p130-binding
Calculated Molecular Weight 44 kDa
GenBank Accession NumberBC033180
Gene Symbol E2F4
Gene ID (NCBI) 1874
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transcription factor E2F4 (E2F4) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.The observed molecular weight of 60-70 kDa is the phosphorylation of E2F4 protein.


Product Specific Protocols
WB protocol for E2F4 antibody 67812-1-IgDownload protocol
Standard Protocols
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