EAPP Polyclonal antibody

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  WB analysis of HEK-293 using 16931-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16931-1-AP (EAPP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16931-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16931-1-AP (EAPP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 16931-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16931-1-AP (EAPP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

EAPP Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 16931-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

E2F-associated phosphoprotein, E2F associated phosphoprotein, C14orf11, BM-036, BM036

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16931-1-AP targets EAPP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10403

  Product name: Recombinant human EAPP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-285 aa of BC001245

  Sequence: MNRLPDDYDPYAVEEPSDEEPALSSSEDEVDVLLHGTPDQKRKLIRECLTGESESSSEDEFEKEMEAELNSTMKTMEDKLSSLGTGSSSGNGKVATAPTRYYDDIYFDSDSEDEDRAVQVTKKKKKKQHKIPTNDELLYDPEKDNRDQAWVDAQRRGYHGLGPQRSREQQPVPNSDAVLNCPACMTTLCLDCQRHESYKTQYRAMFVMNCSINKEEVLRYKASENRKKRRVHKKMRSNQEDAAEKAETDVEEIYHPVMCTECSTEVAVYDKDEVFHFFNVLASHS

• Full Name: E2F-associated phosphoprotein
• Calculated Molecular Weight: 285 aa, 33 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC001245
• Gene Symbol: EAPP
• Gene ID (NCBI): 55837
• RRID: AB_2878334
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q56P03
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.