EBP2 Polyclonal antibody

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EBP2 Polyclonal Antibody for WB, ELISA

Cat No. 15973-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

EBNA1 binding protein 2, EBNA1BP2, EBP2, NOBP, Nucleolar protein p40, P40

• 

  WB analysis of MCF-7 using 15973-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 15973-1-AP (EBP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 15973-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15973-1-AP (EBP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 15973-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15973-1-AP (EBP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of NIH/3T3 using 15973-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15973-1-AP (EBP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 15973-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15973-1-AP (EBP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Raji using 15973-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15973-1-AP (EBP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A549 using 15973-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15973-1-AP (EBP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: MCF-7 cells, HeLa cells, HEK-293 cells, A549 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15973-1-AP targets EBP2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8760

  Product name: Recombinant human EBP2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-306 aa of BC009175

  Sequence: MDTPPLSDSESESDESLVTDRELQDAFSRGLLKPGLNVVLEGPKKAVNDVNGLKQCLAEFKRDLEWVERLDVTLGPVPEIGGSEAPAPQNKDQKAVDPEDDFQREMSFYRQAQAAVLAVLPRLHQLKVPTKRPTDYFAEMAKSDLQMQKIRQKLQTKQAAMERSEKAKQLRALRKYGKKVQTEVLQKRQQEKAHMMNAIKKYQKGFSDKLDFLEGDQKPLAQRKKAGAKGQQMRKGPSAKRRYKNQKFGFGGKKKGSKWNTRESYDDVSSFRAKTAHGRGLKRPGKKGSNKRPGKRTREKMKNRTH

• Full Name: EBNA1 binding protein 2
• Calculated Molecular Weight: 306 aa, 35 kDa
• Observed Molecular Weight: 35-38 kDa
• GenBank Accession Number: BC009175
• Gene Symbol: EBP2
• Gene ID (NCBI): 10969
• RRID: AB_2878204
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99848
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The EBP2 Polyclonal antibody (Cat# 15973-1-AP) has 1 citation. It appears in Molecular Oncology (2020), where it was used in Western blot to study EBP2 as a novel NPM-ALK-interacting nucleolar protein contributing to anaplastic large cell lymphoma (ALCL) cell proliferation through p53 regulation. The associated research field is oncology, specifically lymphoma biology and tumor suppressor mechanisms.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Mol Oncol; EBP2, a novel NPM-ALK-interacting protein in the nucleolus, contributes to the proliferation of ALCL cells by regulating tumor suppressor p53.; Authors - Yuki Uchihara ; KD Validated