ECE1 Polyclonal antibody

ECE1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26088-1-AP

Synonyms

ECE, ECE 1, ECE1, endothelin converting enzyme 1

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Validation Data Gallery

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  WB analysis using 26088-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26088-1-AP (ECE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 26088-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 26088-1-AP (ECE1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26088-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26088-1-AP (ECE1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 26088-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26088-1-AP (ECE1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 26088-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26088-1-AP (ECE1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26088-1-AP is ECE1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ECE1 fusion protein Ag23144
• Full Name: endothelin converting enzyme 1
• Observed molecular weight: 125-135 kDa
• GenBank accession number: BC126257
• Gene symbol: ECE1
• Gene ID (NCBI): 1889
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.