ECE1 Polyclonal antibody

ECE1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26088-1-AP

Synonyms

ECE, ECE 1, ECE1, endothelin converting enzyme 1



Tested Applications

Positive WB detected inHeLa cells, A549 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26088-1-AP is ECE1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ECE1 fusion protein Ag23144
Full Name endothelin converting enzyme 1
Observed molecular weight 125-135 kDa
GenBank accession numberBC126257
Gene symbol ECE1
Gene ID (NCBI) 1889
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ECE1 antibody 26088-1-APDownload protocol
IHC protocol for ECE1 antibody 26088-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols