ECE1 Polyclonal antibody

ECE1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 26088-1-AP

Publications (3)

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

EC:3.4.24.71, ECE, ECE-1, Endothelin-converting enzyme 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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WB analysis using 26088-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 26088-1-AP (ECE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 26088-1-AP
WB result of ECE1 antibody (26088-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-ECE1 transfected HeLa cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of A549 using 26088-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 26088-1-AP (ECE1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 26088-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 26088-1-AP (ECE1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 26088-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26088-1-AP (ECE1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 26088-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26088-1-AP (ECE1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (3)
Protocols
Reviews (1)

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, A549 cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26088-1-AP targets ECE1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag23144 Product name: Recombinant human ECE1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 335-425 aa of BC126257 Sequence: TAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLEQISTLINTTDRCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTK Predict reactive species
Full Name	endothelin converting enzyme 1
Observed Molecular Weight	125-135 kDa
GenBank Accession Number	BC126257
Gene Symbol	ECE1
Gene ID (NCBI)	1889
RRID	AB_2880371
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P42892
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for ECE1 antibody 26088-1-AP	Download protocol
WB protocol for ECE1 antibody 26088-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The antibody 26088-1-AP (anti-ECE1) has 3 total citations. These appear in Advanced Science, Proceedings of the National Academy of Sciences, and Laryngoscope Investigative Otolaryngology. The associated research fields include spinal cord injury and neuroinflammation, Alzheimer's disease (Aβ degradation pathway), and otology (cholesteatoma keratinocyte proliferation). Applications cited are immunofluorescence and Western blot, with species spanning rat and human.

Species	Application	Title
rat	IF	Adv Sci (Weinh) Astrocytic ET-1 System Determines Microglia Phenotype Following Spinal Cord Injury Authors - Bingqiang He View Article
human	WB	Proc Natl Acad Sci U S A Characterization of endothelin-converting enzyme 1 as a key enzyme in the multienzyme Aβ degradation pathway. Authors - Irem Ulku KD Validated View Article
human	WB	Laryngoscope Investig Otolaryngol Hypoxia-inducible factor-1α contributes to the proliferation of cholesteatoma keratinocytes through regulating endothelin converting enzyme 1 expression Authors - Nie Chen KD Validated View Article