ECE1 Polyclonal antibody

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  WB analysis using 26088-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26088-1-AP (ECE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 26088-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 26088-1-AP (ECE1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26088-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26088-1-AP (ECE1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 26088-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26088-1-AP (ECE1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 26088-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26088-1-AP (ECE1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ECE1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 26088-1-AP

Publications(2)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

ECE, ECE 1, ECE1, endothelin converting enzyme 1

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

26088-1-AP targets ECE1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag23144

  Product name: Recombinant human ECE1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 335-425 aa of BC126257

  Sequence: TAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLEQISTLINTTDRCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTK

• Full Name: endothelin converting enzyme 1
• Observed Molecular Weight: 125-135 kDa
• GenBank Accession Number: BC126257
• Gene Symbol: ECE1
• Gene ID (NCBI): 1889
• RRID: AB_2880371
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P42892
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Laryngoscope Investig Otolaryngol; Hypoxia-inducible factor-1α contributes to the proliferation of cholesteatoma keratinocytes through regulating endothelin converting enzyme 1 expression; Authors - Nie Chen ; KD Validated
rat	IF	Adv Sci (Weinh); Astrocytic ET-1 System Determines Microglia Phenotype Following Spinal Cord Injury; Authors - Bingqiang He

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jaouhara (Verified Customer) (12-05-2023)

Specific antibody. Validated in immunofluorescence and immunohistochemistry. Good signal.

• Applications: Immunohistochemistry, Immunofluorescence
• Cell Tissue Type: Chondrocytes