Tested Applications
| Positive WB detected in | HeLa cells, A549 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26088-1-AP targets ECE1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23144 Product name: Recombinant human ECE1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 335-425 aa of BC126257 Sequence: TAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLEQISTLINTTDRCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTK Predict reactive species |
| Full Name | endothelin converting enzyme 1 |
| Observed Molecular Weight | 125-135 kDa |
| GenBank Accession Number | BC126257 |
| Gene Symbol | ECE1 |
| Gene ID (NCBI) | 1889 |
| RRID | AB_2880371 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42892 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ECE1 antibody 26088-1-AP | Download protocol |
| WB protocol for ECE1 antibody 26088-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ECE1 Polyclonal antibody (catalog number 26088-1-AP) has 3 total citations. These appear in Advanced Science, Proceedings of the National Academy of Sciences (PNAS), and Laryngoscope Investigative Otolaryngology. The associated research fields include spinal cord injury and microglia phenotype, Aβ degradation pathway, and cholesteatoma keratinocyte proliferation. Cited applications include Western blotting and immunofluorescence, with human and rat reactivity.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Astrocytic ET-1 System Determines Microglia Phenotype Following Spinal Cord Injury | ||
Proc Natl Acad Sci U S A Characterization of endothelin-converting enzyme 1 as a key enzyme in the multienzyme Aβ degradation pathway.
| ||
Laryngoscope Investig Otolaryngol Hypoxia-inducible factor-1α contributes to the proliferation of cholesteatoma keratinocytes through regulating endothelin converting enzyme 1 expression
|
Reviews
What customers say
The single review for this antibody reports a 5-star rating. The user validated the product in both immunofluorescence and immunohistochemistry on chondrocytes, describing it as a specific antibody that produced good signal. Overall, the feedback is positive, highlighting reliable specificity and strong performance across two applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jaouhara (Verified Customer) (12-05-2023) | Specific antibody. Validated in immunofluorescence and immunohistochemistry. Good signal.
|
