ECE2 Polyclonal antibody

ECE2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15418-1-AP

Synonyms

ECE2, endothelin converting enzyme 2, KIAA0604



Tested Applications

Positive WB detected inmouse liver tissue, mouse lung tissue, mouse ovary tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

15418-1-AP targets ECE2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ECE2 fusion protein Ag7706
Full Name endothelin converting enzyme 2
Calculated molecular weight 100 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC005835
Gene symbol ECE2
Gene ID (NCBI) 9718
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Endothelin-converting enzymes (ECEs) are the key enzymes in the endothelin (ET) biosynthesis that catalyze the conversion of big ET, the biologically inactive precursor of mature ET. Two enzymes, termed ECE-1 and ECE-2, have been molecularly identified. ECE2(Endothelin-converting enzyme 2) is also named as KIAA0604 and belongs to the methyltransferase superfamily in the N-terminal section, belongs to the peptidase M13 family in the C-terminal section. It is able to process endoproteolytically peptides intermediates to generate biologically active peptides.

Protocols

Product Specific Protocols
WB protocol for ECE2 antibody 15418-1-APDownload protocol
IHC protocol for ECE2 antibody 15418-1-APDownload protocol
IP protocol for ECE2 antibody 15418-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

PLoS One

Proteolysis controls endogenous substance p levels.

Authors - Mitchell Andrew J AJ