ECE2 Polyclonal antibody

ECE2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15418-1-AP

Synonyms

ECE2, endothelin converting enzyme 2, KIAA0604

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Validation Data Gallery

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  WB analysis of mouse liver using 15418-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15418-1-AP (ECE2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 15418-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 15418-1-AP (ECE2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 15418-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15418-1-AP (ECE2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 15418-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15418-1-AP (ECE2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15418-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15418-1-AP (ECE2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 15418-1-AP

  IP Result of anti-ECE2 (IP:15418-1-AP, 4ug; Detection:15418-1-AP 1:500) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human liver using 15418-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15418-1-AP (ECE2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 15418-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15418-1-AP (ECE2 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue, mouse lung tissue, mouse ovary tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:100-1:400

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below

Product Information

15418-1-AP targets ECE2 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ECE2 fusion protein Ag7706
• Full Name: endothelin converting enzyme 2
• Calculated molecular weight: 100 kDa
• Observed molecular weight: 100 kDa
• GenBank accession number: BC005835
• Gene symbol: ECE2
• Gene ID (NCBI): 9718
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Endothelin-converting enzymes (ECEs) are the key enzymes in the endothelin (ET) biosynthesis that catalyze the conversion of big ET, the biologically inactive precursor of mature ET. Two enzymes, termed ECE-1 and ECE-2, have been molecularly identified. ECE2(Endothelin-converting enzyme 2) is also named as KIAA0604 and belongs to the methyltransferase superfamily in the N-terminal section, belongs to the peptidase M13 family in the C-terminal section. It is able to process endoproteolytically peptides intermediates to generate biologically active peptides.

Publications

Species	Application	Title
mouse	WB	PLoS One; Proteolysis controls endogenous substance p levels.; Authors - Mitchell Andrew J AJ