Tested Applications
| Positive WB detected in | mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15418-1-AP targets EEF1AKMT4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7706 Product name: Recombinant human ECE2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-255 aa of BC005835 Sequence: MASPGAGRAPPELPERNCGYREVEYWDQRYQGAADSAPYDWFGDFSSFRALLEPELRPEDRILVLGCGNSALSYELFLGGFPNVTSVDYSSVVVAAMQARYAHVPQLRWETMDVRKLDFPSASFDVVLEKGTLDALLAGERDPWTVSSEGVHTVDQVLSEVSRVLVPGGRFISMTSAAPHFRTRHYAQAYYGWSLRHATYGSGFHFHLYLMHKGGKLSVAQLALGAQILSPPRPPTSPCFLQDSDHEDFLSAIQL Predict reactive species |
| Full Name | EEF1A lysine methyltransferase 4 |
| Calculated Molecular Weight | 28 kDa |
| Observed Molecular Weight | 28 kDa |
| GenBank Accession Number | BC005835 |
| Gene Symbol | EEF1AKMT4 |
| Gene ID (NCBI) | 110599564 |
| RRID | AB_2878134 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P0DPD7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EEF1AKMT4 antibody 15418-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The EEF1AKMT4 Polyclonal antibody (catalog 15418-1-AP) has 2 citations. They appear in Genes Dis and PLoS One. Research fields include gallbladder cancer (GBC) progression via ribosomal protein output (human, WB) and proteolysis control of endogenous substance P levels (mouse, WB).
| Species | Application | Title |
|---|---|---|
Genes Dis EEF1AKMT4-eEF1A2 synergistically facilitates the progression of GBC by promoting ribosomal protein output.
| ||
Reviews
What customers say
The single review for this product is a 5-star rating. The user performed immunohistochemistry and immunofluorescence on paraffin-fixed human chondrocytes and reported a good, specific signal. Overall, the feedback is positive, with the reviewer highlighting the antibody's strong specificity and clear staining performance in their application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jaouhara (Verified Customer) (12-05-2023) | Immunohistochemical analysis of paraffin-fixed human chondrocytes using 15418-1-AP (ECE2 antibody). Good and sepcific signal.
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