ECH1 Polyclonal antibody

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• ECH1 Polyclonal antibody (11385-1-AP)

ECH1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11385-1-AP Copied!

Publications (11)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

HPXEL, EC:5.3.3.-, Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial

• 

  WB analysis of mouse liver using 11385-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 11385-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse heart using 11385-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse heart using 11385-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of L02 using 11385-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 11385-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 11385-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 11385-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11385-1-AP (ECH1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse liver tissue, HepG2 cells, Jurkat cells, K-562 cells, L02 cells, mouse heart tissue
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11385-1-AP targets ECH1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1931

  Product name: Recombinant human ECH1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-328 aa of BC017408

  Sequence: MAAGIVASRRLRDLLTRRLTGSNYPGLSISLRLTGSSAQEAASGVALGEAPDHSYESLRVTSAQKHVLHVQLNRPNKRNAMNKVFWREMVECFNKISRDADCRAVVISGAGKMFTAGIDLMDMASDILQPKGDDVARISWYLRDIITRYQETFNVIERCPKPVIAAVHGGCIGGGVDLVTACDIRYCAQDAFFQVKEVDVGLAADVGTLQRLPKVIGNQSLVNELAFTARKMMADEALGSGLVSRVFPDKEVMLDAALALAAEISSKSPVAVQSTKVNLLYSRDHSVAESLNYVASWNMSMLQTQDLVKSVQATTENKELKTVTFSKL

• Full Name: enoyl Coenzyme A hydratase 1, peroxisomal
• Calculated Molecular Weight: 328 aa, 36 kDa
• Observed Molecular Weight: 30-36 kDa
• GenBank Accession Number: BC017408
• Gene Symbol: ECH1
• Gene ID (NCBI): 1891
• RRID: AB_2277810
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13011
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Enoyl coenzyme A hydratase 1 (ECH1), is first recog-nized as a key component in mitochondrial fatty acid β-oxidation, and subsequent studies have demonstrated that it regulates multiple pathophysiological processes (PMID:33813878). The MW of ECH1 is 36 kDa,and can be detected as 30-36 kDa.

Publications

What published studies show

The ECH1 polyclonal antibody (Cat# 11385-1-AP) has 11 total citations published in journals including Cell, Cell Metabolism, Cancer Research, Hepatology, Journal of Clinical Investigation, Cell Death & Differentiation, Geroscience, Frontiers in Cell and Developmental Biology, ACS Omega, European Journal of Immunology, and Biochemical and Biophysical Research Communications. Associated research fields span lipid metabolism, cancer biology, metabolic disease, immunology, mitochondrial function, and aging.

Automatically generated from the 11 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell; ACSL4 and polyunsaturated lipids support metastatic extravasation and colonization; Authors - Yuqi Wang
mouse	WB	Cell Metab; Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity; Authors - Zilong Zhang
		Cancer Res; Long-Chain Fatty Acid Redistribution Induced by SLC27A2 Deficiency Facilitates Hypoxic Adaptation and Immunosuppression in Hepatocellular Carcinoma; Authors - Chuli Fu
mouse	WB	Hepatology; High fat and high sucrose (western) diet induce steatohepatitis that is dependent on fructokinase.; Authors - Ishimoto Takuji T
mouse	WB	J Clin Invest; Ketohexokinase C blockade ameliorates fructose-induced metabolic dysfunction in fructose-sensitive mice.; Authors - Miguel A Lanaspa
human	WB	Cell Death Differ; C-IGF1R encoded by cIGF1R acts as a molecular switch to restrict mitophagy of drug-tolerant persister tumour cells in non-small cell lung cancer; Authors - Hui Wang