ECH1 Polyclonal antibody

ECH1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11385-1-AP

Synonyms

ECH1, HPXEL



Tested Applications

Positive WB detected inmouse liver tissue, HepG2 cells, Jurkat cells, K-562 cells, L02 cells, mouse heart tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

11385-1-AP targets ECH1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ECH1 fusion protein Ag1931
Full Name enoyl Coenzyme A hydratase 1, peroxisomal
Calculated molecular weight 328 aa, 36 kDa
Observed molecular weight 30-36 kDa
GenBank accession numberBC017408
Gene symbol ECH1
Gene ID (NCBI) 1891
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ECH1 antibody 11385-1-APDownload protocol
IHC protocol for ECH1 antibody 11385-1-APDownload protocol
IF protocol for ECH1 antibody 11385-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Eur J Immunol

YY1 control of mitochondrial-related genes does not account for regulation of immunoglobulin class switch recombination in mice.

Authors - Satabdi Nandi
mouseWB

Hepatology

High fat and high sucrose (western) diet induce steatohepatitis that is dependent on fructokinase.

Authors - Ishimoto Takuji T
mouseWB

J Clin Invest

Ketohexokinase C blockade ameliorates fructose-induced metabolic dysfunction in fructose-sensitive mice.

Authors - Miguel A Lanaspa