ECH1 Monoclonal antibody, PBS Only

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  WB analysis using 68139-1-Ig (same clone as 68139-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68139-1-Ig (ECH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68139-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 68139-1-Ig (same clone as 68139-1-PBS)

  WB result of ECH1 antibody (68139-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ECH1 transfected HepG2 cells. This data was developed using the same antibody clone with 68139-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 68139-1-Ig (same clone as 68139-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68139-1-Ig (ECH1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68139-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 68139-1-Ig (same clone as 68139-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68139-1-Ig (ECH1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68139-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68139-1-Ig (same clone as 68139-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ECH1 antibody (68139-1-Ig, Clone: 1C9G5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68139-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

ECH1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 68139-1-PBS

Clone No.1C9G5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

1C9G5, Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial, EC:5.3.3.-, HPXEL

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Product Information

68139-1-PBS targets ECH1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag32661

  Product name: Recombinant human ECH1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-328 aa of BC017408

  Sequence: MAAGIVASRRLRDLLTRRLTGSNYPGLSISLRLTGSSAQEAASGVALGEAPDHSYESLRVTSAQKHVLHVQLNRPNKRNAMNKVFWREMVECFNKISRDADCRAVVISGAGKMFTAGIDLMDMASDILQPKGDDVARISWYLRDIITRYQETFNVIERCPKPVIAAVHGGCIGGGVDLVTACDIRYCAQDAFFQVKEVDVGLAADVGTLQRLPKVIGNQSLVNELAFTARKMMADEALGSGLVSRVFPDKEVMLDAALALAAEISSKSPVAVQSTKVNLLYSRDHSVAESLNYVASWNMSMLQTQDLVKSVQATTENKELKTVTFSKL

• Full Name: enoyl Coenzyme A hydratase 1, peroxisomal
• Calculated Molecular Weight: 328 aa, 36 kDa
• Observed Molecular Weight: 33-36 kDa
• GenBank Accession Number: BC017408
• Gene Symbol: ECH1
• Gene ID (NCBI): 1891
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q13011
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.