ECHDC1 Polyclonal antibody

ECHDC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27732-1-AP

Synonyms

ECHDC1

---

Validation Data Gallery

• 

  WB analysis of PC-12 using 27732-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 27732-1-AP (ECHDC1 antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of A549 using 27732-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27732-1-AP (ECHDC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 27732-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 27732-1-AP (ECHDC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 27732-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27732-1-AP (ECHDC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 27732-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27732-1-AP (ECHDC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 27732-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27732-1-AP (ECHDC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 27732-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27732-1-AP (ECHDC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: PC-12 cells, A549 cells
• Positive IHC detected in: mouse kidney tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27732-1-AP targets ECHDC1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ECHDC1 fusion protein Ag26905
• Full Name: enoyl Coenzyme A hydratase domain containing 1
• Calculated Molecular Weight: 307 aa, 34 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC003549
• Gene Symbol: ECHDC1
• Gene ID (NCBI): 55862
• RRID: AB_2880956
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.