ECHS1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 81611-1-RR (same clone as 81611-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81611-1-RR (ECHS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81611-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 81611-1-RR (same clone as 81611-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81611-1-RR (ECHS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81611-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 81611-1-RR (same clone as 81611-1-PBS)

  WB result of ECHS1 antibody (81611-1-RR; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ECHS1 transfected MCF-7 cells. This data was developed using the same antibody clone with 81611-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 81611-1-RR (same clone as 81611-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 81611-1-RR (ECHS1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81611-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 81611-1-RR (same clone as 81611-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 81611-1-RR (ECHS1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81611-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 81611-1-RR (same clone as 81611-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ECHS1 antibody (81611-1-RR, Clone: 5G5 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 81611-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81611-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81611-1-RR against Human ECHS1 were performed. The affinity constant is 95.5 pM.

• Featured Product
• KD/KO Validated

ECHS1 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 81611-1-PBS

Clone No.5G5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

mECH, Enoyl-CoA hydratase, mitochondrial, Enoyl-CoA hydratase 1, ECHM, EC:4.2.1.17

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Product Information

81611-1-PBS targets ECHS1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1842

  Product name: Recombinant human ECHS1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-290 aa of BC008906

  Sequence: MAALRVLLSCVRGPLRPPVRCPAWRPFASGANFEYIIAEKRGKNNTVGLIQLNRPKALNALCDGLIDELNQALKIFEEDPAVGAIVLTGGDKAFAAGADIKEMQNLSFQDCYSSKFLKHWDHLTQVKKPVIAAVNGYAFGGGCELAMMCDIIYAGEKAQFAQPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKICPVETLVEEAIQCAEKIASNSKIVVAMAKESVNAAFEMTLTEGSKLEKKLFYSTFATDDRKEGMTAFVEKRKANFKDQ

• Full Name: enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC008906
• Gene Symbol: ECHS1
• Gene ID (NCBI): 1892
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P30084
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Enoyl-coenzyme A hydratase (ECHS1) is a mitochondrial protein which catalyzes the hydration of 2-trans-enoyl-coenzyme A intermediates to L-3-hydroxyacyl-coenzyme A, playing a key role in metabolism of fatty acids in mitochondria. ECHS1 is highly expressed in muscle, liver and fibroblasts. Altered expression of ECHS1 has been considered a characteristic feature of mitochondria dysfunction. (23275097, 23235493)