ECSIT Polyclonal antibody

ECSIT Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG







Cat no : 27548-1-AP


ECSIT, ECSIT homolog (Drosophila), Protein SITPEC, SITPEC

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27548-1-AP targets ECSIT in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ECSIT fusion protein Ag26877
Full Name ECSIT homolog (Drosophila)
Calculated Molecular Weight 49 kDa
Observed Molecular Weight49 kDa
GenBank Accession NumberBC008279
Gene Symbol ECSIT
Gene ID (NCBI) 51295
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ECSIT is a cytosolic adaptor protein associated with the toll-like receptor pathway. It has a distinct N-terminal mitochondrial targeting sequence, pentatricopeptide repeat motif, and a C-terminal pleckstrin homology domain. ECSIT regulates many biological processes like embryonic development, inflammation, cardiac function, and assembly of mitochondrial complex I. In macrophages, the activation of TLRs by inflammatory signals promotes the translocation of TRAF6 to mitochondria where it engages ECSIT to stimulate the production of mitochondrial reactive oxygen species (ROS).


Product Specific Protocols
WB protocol for ECSIT antibody 27548-1-APDownload protocol
Standard Protocols
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