EDAR Polyclonal antibody

EDAR Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 18032-1-AP

Synonyms

DL, Downless homolog, Ectodermal dysplasia receptor, ectodysplasin A receptor, ED1R, ED3, ED5, EDA A1 receptor, EDA A1R, EDA1R, EDA3, EDAR, HRM1

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Validation Data Gallery

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  WB analysis of A549 using 18032-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18032-1-AP (EDAR antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis using 18032-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18032-1-AP (EDAR antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 18032-1-AP

  IP result of anti-EDAR (IP:18032-1-AP, 4ug; Detection:18032-1-AP 1:500) with A549 cells lysate 2800ug.

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, A431 cells, MCF-7 cells
• Positive IP detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

18032-1-AP targets EDAR in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EDAR fusion protein Ag12601
• Full Name: ectodysplasin A receptor
• Calculated Molecular Weight: 448 aa, 49 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC093872
• Gene Symbol: EDAR
• Gene ID (NCBI): 10913
• RRID: AB_10596932
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EDAR, also named as DL, is a receptor for EDA isoform A1, but not for EDA isoform A2. It mediates the activation of NF-kappa-B and JNK. EDAR may promote caspase-independent cell death. It is expressed in adjacent or partially overlapping layers in the developing human skin with MW 48 kDa.

Publications

Species	Application	Title
mouse	IF	Development; Involvement of Wnt, Eda and Shh at defined stages of sweat gland development.; Authors - Chang-Yi Cui
mouse	WB	J Pharm Pharmacol; Minimally modified low-density lipoprotein upregulates the ETB and α1 receptors in mouse mesenteric arteries in vivo by activating the PI3K/Akt pathway.; Authors - Zhong-San Zeng
mouse	IF,IHC	J Histochem Cytochem; Spatial and Temporal Expression of Ectodysplasin-A Signaling Pathway Members During Mandibular Condylar Development in Postnatal Mice; Authors - Ludan Xing
mouse	WB	J Invest Dermatol; ASH2L mediates epidermal differentiation and hair follicle morphogenesis via H3K4me3 modification; Authors - Qirui Wang