EDIL3 Polyclonal antibody

• Featured Product
• KD/KO Validated

EDIL3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

18

Reviews (1)

Cat no : 12580-1-AP

Synonyms

Integrin-binding protein DEL1, EGF-like repeat and discoidin I-like domain-containing protein 3, Developmentally-regulated endothelial cell locus 1 protein, DEL1

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Validation Data Gallery

• 

  WB analysis using 12580-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12580-1-AP (EDIL3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 12580-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12580-1-AP (EDIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12580-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12580-1-AP (EDIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12580-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12580-1-AP (EDIL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 12580-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12580-1-AP (EDIL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12580-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12580-1-AP (EDIL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 12580-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12580-1-AP (EDIL3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12580-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12580-1-AP (EDIL3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12580-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12580-1-AP (EDIL3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 12580-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12580-1-AP (EDIL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12580-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12580-1-AP (EDIL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12580-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12580-1-AP (EDIL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12580-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12580-1-AP (EDIL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12580-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 12580-1-AP (EDIL3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 12580-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 12580-1-AP (EDIL3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 12580-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 12580-1-AP (EDIL3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, COLO 320 cells, mouse brain tissue, HepG2 cells, mouse lung tissue, human lung tissue, HeLa cells, K-562 cells
• Positive IHC detected in: human pancreas cancer tissue, human lung cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 8 publications below
• IHC: See 5 publications below
• IF: See 4 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Product Information

12580-1-AP targets EDIL3 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EDIL3 fusion protein Ag3274
• Full Name: EGF-like repeats and discoidin I-like domains 3
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 52-55 kDa
• GenBank Accession Number: BC030828
• Gene Symbol: EDIL3
• Gene ID (NCBI): 10085
• RRID: AB_2096219
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EGF-like repeats and discoidin I-like domain-containing protein 3 (EDIL3), also named as DEL1 or integrin-biding protein DEL1, is a 52-kDa extracellular matrix protein produced by endothelial cells in embryos (PMID: 9420328). It is composed of three EGF repeats and two discoidin I-like domains. The second EGF repeat contains an RGD motif. EDIL3 promotes adhesion of endothelial cells through interaction with the alpha-v/beta-3 integrin receptor. It may be involved in vascular remodeling during angiogenesis (PMID: 12840057; 14981004). EDIL3 has also been reported to be an endogenous inhibitor of inflammatory cell recruitment by interfering with the integrin LFA-1-dependent leukocyte-endothelial adhesion (PMID: 19008446).

Publications

Species	Application	Title
mouse	IF	J Clin Invest; Secreted protein Del-1 regulates myelopoiesis in the hematopoietic stem cell niche.; Authors - Ioannis Mitroulis ; KO Validated
human	ELISA	Nat Commun; Antagonistic effects of IL-17 and D-resolvins on endothelial Del-1 expression through a GSK-3β-C/EBPβ pathway.; Authors - Tomoki Maekawa
human	WB,IHC	Oncotarget; Overexpressed EDIL3 predicts poor prognosis and promotes anchorage-independent tumor growth in human pancreatic cancer.; Authors - Shu-Heng Jiang ; KD Validated
		Thromb Haemost; Developmental endothelial locus-1 attenuates complement-dependent phagocytosis through inhibition of Mac-1-integrin.; Authors - Ioannis Mitroulis
		Sci Rep; Inhibition of CDK9 as a therapeutic strategy for inflammatory arthritis.; Authors - Annelie Hellvard
human	WB, IHC	Mol Cancer; Elevated autocrine EDIL3 protects hepatocellular carcinoma from anoikis through RGD-mediated integrin activation.; Authors - Ming-Xuan Feng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Guo-rong (Verified Customer) (08-30-2022)

It gives a clear and specific band

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: ARPE-19

EDIL3 Antibody Western Blot validation (1:500 dilution) in ARPE-19 (Cat no:12580-1-AP)

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