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- KD/KO Validated
EEF1A2 Polyclonal antibody
EEF1A2 Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IP, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 16091-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF7 cells, human brain tissue, MCF-7 cells, mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 8 publications below |
| IHC | See 3 publications below |
| IF | See 4 publications below |
Product Information
16091-1-AP targets EEF1A2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, oyster, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | EEF1A2 fusion protein Ag6875 |
| Full Name | eukaryotic translation elongation factor 1 alpha 2 |
| Calculated molecular weight | 50 kDa |
| Observed molecular weight | 50-53 kDa |
| GenBank accession number | BC000432 |
| Gene symbol | EEF1A2 |
| Gene ID (NCBI) | 1917 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EEF1A2, also named as EEF1AL, STN and Statin-S1, belongs to the GTP-binding elongation factor family and EF-Tu/EF-1A subfamily. It promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. EEF1A2 may be critical in the development of ovarian cancer. The MW of EEF1A2 is 50-53kd. This antibody can recognize both EEF1A1 and EEF1A2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EEF1A2 antibody 16091-1-AP | Download protocol |
| IHC protocol for EEF1A2 antibody 16091-1-AP | Download protocol |
| IP protocol for EEF1A2 antibody 16091-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Kaohsiung J Med Sci Molecular markers associated with nonepithelial ovarian cancer in formalin-fixed, paraffin-embedded specimens by genome wide expression profiling. | ||
Biosci Rep Translation elongation factor 1A2 is encoded by one of four closely related eef1a genes and is dispensable for survival in zebrafish. | ||
J Pharm Biomed Anal A rapid and specific method to simultaneously quantify eukaryotic elongation factor 1A1 and A2 protein levels in cancer cells.
| ||
Ann Transl Med Peptidome characterization of ovarian cancer serum and the identification of tumor suppressive peptide ZYX36-58. | ||
Proteomics A comparative proteomic analysis reveals important proteins for the fertilization and early embryonic development of the oyster Crassostrea gigas. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH WEI (Verified Customer) (01-17-2020) | KO validated by KO mice, very good for western blot and immunofluorescence
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