|Positive WB detected in
|HEK-293 cells, MCF-7 cells, mouse brain tissue, mouse heart tissue, mouse skeletal muscle tissue, rat brain tissue
|Positive IP detected in
|mouse brain tissue
|Positive IHC detected in
|human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:2000-1:10000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:20-1:200
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
16091-1-AP targets EEF1A2 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat, oyster, zebrafish
|Host / Isotype
|Rabbit / IgG
|EEF1A2 fusion protein Ag6875
|eukaryotic translation elongation factor 1 alpha 2
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
EEF1A2, also named as EEF1AL, STN and Statin-S1, belongs to the GTP-binding elongation factor family and EF-Tu/EF-1A subfamily. It promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. EEF1A2 may be critical in the development of ovarian cancer. The MW of EEF1A2 is 50-53kd. This antibody can recognize both EEF1A1 and EEF1A2.
EEF1A1 deacetylation enables transcriptional activation of remyelination.
SNX16 activates c-Myc signaling by inhibiting ubiquitin-mediated proteasomal degradation of eEF1A2 in colorectal cancer development.
Nannocystin ax, an eEF1A inhibitor, induces G1 cell cycle arrest and caspase-independent apoptosis through cyclin D1 downregulation in colon cancer in vivo.
Biallelic mutations in the gene encoding eEF1A2 cause seizures and sudden death in F0 mice.
Br J Cancer
Dissecting the expression of EEF1A1/2 genes in human prostate cancer cells: the potential of EEF1A2 as a hallmark for prostate transformation and progression.
Hum Mol Genet
Recapitulation of the EEF1A2 D252H neurodevelopmental disorder-causing missense mutation in mice reveals a toxic gain of function.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
WEI (Verified Customer) (01-17-2020)
KO validated by KO mice, very good for western blot and immunofluorescence