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  • KD/KO Validated

EEF1A2 Polyclonal antibody

EEF1A2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 16091-1-AP

Synonyms

eEF1A 2, EEF1A2, EEF1AL, EF 1 alpha 2, EF1A, Elongation factor 1 alpha 2, HS1, Statin S1, STN, STNL



Tested Applications

Positive WB detected inMCF7 cells, human brain tissue, MCF-7 cells, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

16091-1-AP targets EEF1A2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, oyster, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EEF1A2 fusion protein Ag6875
Full Name eukaryotic translation elongation factor 1 alpha 2
Calculated molecular weight 50 kDa
Observed molecular weight 50-53 kDa
GenBank accession numberBC000432
Gene symbol EEF1A2
Gene ID (NCBI) 1917
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EEF1A2, also named as EEF1AL, STN and Statin-S1, belongs to the GTP-binding elongation factor family and EF-Tu/EF-1A subfamily. It promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. EEF1A2 may be critical in the development of ovarian cancer. The MW of EEF1A2 is 50-53kd. This antibody can recognize both EEF1A1 and EEF1A2.

Protocols

Product Specific Protocols
WB protocol for EEF1A2 antibody 16091-1-APDownload protocol
IHC protocol for EEF1A2 antibody 16091-1-APDownload protocol
IP protocol for EEF1A2 antibody 16091-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Kaohsiung J Med Sci

Molecular markers associated with nonepithelial ovarian cancer in formalin-fixed, paraffin-embedded specimens by genome wide expression profiling.

Authors - Vui-Kee Koon K
zebrafishWB

Biosci Rep

Translation elongation factor 1A2 is encoded by one of four closely related eef1a genes and is dispensable for survival in zebrafish.

Authors - Nwamaka J Idigo
humanWB,IHC,IF

J Pharm Biomed Anal

A rapid and specific method to simultaneously quantify eukaryotic elongation factor 1A1 and A2 protein levels in cancer cells.

Authors - Alessandra Bosutti
  • KD Validated
humanWB

Ann Transl Med

Peptidome characterization of ovarian cancer serum and the identification of tumor suppressive peptide ZYX36-58.

Authors - Xusu Wang
oysterWB,IF

Proteomics

A comparative proteomic analysis reveals important proteins for the fertilization and early embryonic development of the oyster Crassostrea gigas.

Authors - Xiaohong Huang
humanWB

Sci Rep

Comparison of the sensitivity of Western blotting between PVDF and NC membranes.

Authors - Yufang Xiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

WEI (Verified Customer) (01-17-2020)

KO validated by KO mice, very good for western blot and immunofluorescence

  • Applications: Western Blot,Immunofluorescence,
  • Cell Tissue Type: Mouse heart