EEF1A1/EEF1A2 Polyclonal antibody

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  WB analysis using 16091-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16091-1-AP (EEF1A2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16091-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16091-1-AP (EEF1A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 16091-1-AP

  IP result of anti-EEF1A2 (IP:16091-1-AP, 4ug; Detection:16091-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human colon using 16091-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 16091-1-AP (EEF1A2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

EEF1A1/EEF1A2 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 16091-1-AP

Publications(22)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, IP, RIP, ELISA

Synonyms

EEF1A2, eEF1A-2, EEF1AL, EF-1-alpha-2, Elongation factor 1-alpha 2

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Tested Applications

• Positive WB detected in: HEK-293 cells, MCF-7 cells, mouse brain tissue, mouse heart tissue, mouse skeletal muscle tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human colon; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 15 publications below
• IHC: See 5 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Product Information

16091-1-AP targets EEF1A1/EEF1A2 in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, zebrafish, oyster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EEF1A1/EEF1A2 fusion protein Ag6875
• Full Name: eukaryotic translation elongation factor 1 alpha 2
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50-53 kDa
• GenBank Accession Number: BC000432
• Gene Symbol: EEF1A2
• Gene ID (NCBI): 1917
• RRID: AB_2096979
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q05639
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EEF1A2, also named as EEF1AL, STN and Statin-S1, belongs to the GTP-binding elongation factor family and EF-Tu/EF-1A subfamily. It promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. EEF1A2 may be critical in the development of ovarian cancer. The MW of EEF1A2 is 50-53kd. This antibody can recognize both EEF1A1 and EEF1A2.

Publications

Species	Application	Title
		Nat Commun; EEF1A1 deacetylation enables transcriptional activation of remyelination.; Authors - Mert Duman
mouse,human	WB,IHC,IF	Mol Oncol; SNX16 activates c-Myc signaling by inhibiting ubiquitin-mediated proteasomal degradation of eEF1A2 in colorectal cancer development.; Authors - Zhiyong Shen
human	WB	Pharmacol Res; Nannocystin ax, an eEF1A inhibitor, induces G1 cell cycle arrest and caspase-independent apoptosis through cyclin D1 downregulation in colon cancer in vivo.; Authors - Ying Hou
mouse	WB	Sci Rep; Biallelic mutations in the gene encoding eEF1A2 cause seizures and sudden death in F0 mice.; Authors - Faith C J Davies ; KO Validated
human	IF	Br J Cancer; Dissecting the expression of EEF1A1/2 genes in human prostate cancer cells: the potential of EEF1A2 as a hallmark for prostate transformation and progression.; Authors - Scaggiante B B
mouse	WB	Hum Mol Genet; Recapitulation of the EEF1A2 D252H neurodevelopmental disorder-causing missense mutation in mice reveals a toxic gain of function.; Authors - Faith C J Davies

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

WEI (Verified Customer) (01-17-2020)

KO validated by KO mice, very good for western blot and immunofluorescence

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: Mouse heart