• Featured Product
  • KD/KO Validated

EEF1A2 Polyclonal antibody

EEF1A2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16091-1-AP

Synonyms

eEF1A 2, EEF1A2, EEF1AL, EF 1 alpha 2, EF1A, Elongation factor 1 alpha 2, HS1, Statin S1, STN, STNL



Tested Applications

Positive WB detected inHEK-293 cells, MCF-7 cells, mouse brain tissue, mouse heart tissue, mouse skeletal muscle tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

16091-1-AP targets EEF1A2 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, oyster, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EEF1A2 fusion protein Ag6875
Full Name eukaryotic translation elongation factor 1 alpha 2
Calculated molecular weight 50 kDa
Observed molecular weight 50-53 kDa
GenBank accession numberBC000432
Gene symbol EEF1A2
Gene ID (NCBI) 1917
RRIDAB_2096979
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EEF1A2, also named as EEF1AL, STN and Statin-S1, belongs to the GTP-binding elongation factor family and EF-Tu/EF-1A subfamily. It promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. EEF1A2 may be critical in the development of ovarian cancer. The MW of EEF1A2 is 50-53kd. This antibody can recognize both EEF1A1 and EEF1A2.

Protocols

Product Specific Protocols
WB protocol for EEF1A2 antibody 16091-1-APDownload protocol
IHC protocol for EEF1A2 antibody 16091-1-APDownload protocol
IP protocol for EEF1A2 antibody 16091-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Nat Commun

EEF1A1 deacetylation enables transcriptional activation of remyelination.

Authors - Mert Duman
human,mouseWB,IHC,IF

Mol Oncol

SNX16 activates c-Myc signaling by inhibiting ubiquitin-mediated proteasomal degradation of eEF1A2 in colorectal cancer development.

Authors - Zhiyong Shen
humanWB

Pharmacol Res

Nannocystin ax, an eEF1A inhibitor, induces G1 cell cycle arrest and caspase-independent apoptosis through cyclin D1 downregulation in colon cancer in vivo.

Authors - Ying Hou
mouseWB

Sci Rep

Biallelic mutations in the gene encoding eEF1A2 cause seizures and sudden death in F0 mice.

Authors - Faith C J Davies
  • KO Validated
humanIF

Br J Cancer

Dissecting the expression of EEF1A1/2 genes in human prostate cancer cells: the potential of EEF1A2 as a hallmark for prostate transformation and progression.

Authors - Scaggiante B B
mouseWB

Hum Mol Genet

Recapitulation of the EEF1A2 D252H neurodevelopmental disorder-causing missense mutation in mice reveals a toxic gain of function.

Authors - Faith C J Davies

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

WEI (Verified Customer) (01-17-2020)

KO validated by KO mice, very good for western blot and immunofluorescence

  • Applications: Western Blot, Immunofluorescence,
  • Cell Tissue Type: Mouse heart