|Positive WB detected in||MCF7 cells, human brain tissue, MCF-7 cells, mouse brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
16091-1-AP targets EEF1A2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, oyster, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||EEF1A2 fusion protein Ag6875|
|Full Name||eukaryotic translation elongation factor 1 alpha 2|
|Calculated molecular weight||50 kDa|
|Observed molecular weight||50-53 kDa|
|GenBank accession number||BC000432|
|Gene ID (NCBI)||1917|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
EEF1A2, also named as EEF1AL, STN and Statin-S1, belongs to the GTP-binding elongation factor family and EF-Tu/EF-1A subfamily. It promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. EEF1A2 may be critical in the development of ovarian cancer. The MW of EEF1A2 is 50-53kd. This antibody can recognize both EEF1A1 and EEF1A2.
Kaohsiung J Med Sci
Molecular markers associated with nonepithelial ovarian cancer in formalin-fixed, paraffin-embedded specimens by genome wide expression profiling.
Translation elongation factor 1A2 is encoded by one of four closely related eef1a genes and is dispensable for survival in zebrafish.
J Pharm Biomed Anal
A rapid and specific method to simultaneously quantify eukaryotic elongation factor 1A1 and A2 protein levels in cancer cells.
Ann Transl Med
Peptidome characterization of ovarian cancer serum and the identification of tumor suppressive peptide ZYX36-58.
A comparative proteomic analysis reveals important proteins for the fertilization and early embryonic development of the oyster Crassostrea gigas.
Comparison of the sensitivity of Western blotting between PVDF and NC membranes.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
WEI (Verified Customer) (01-17-2020)
KO validated by KO mice, very good for western blot and immunofluorescence