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EEF1D Polyclonal antibody
EEF1D Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 10630-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, MCF-7 cells, U-87 MG cells, U2OS cells, NIH/3T3 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human pancreas cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 2 publications below |
WB | See 6 publications below |
CoIP | See 1 publications below |
Product Information
10630-1-AP targets EEF1D in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | EEF1D fusion protein Ag0983 |
Full Name | eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) |
Calculated Molecular Weight | 31 kDa |
Observed Molecular Weight | 35-40 kDa |
GenBank Accession Number | BC007847 |
Gene Symbol | EEF1D |
Gene ID (NCBI) | 1936 |
RRID | AB_2230993 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EEF1D, also named as EF1D and EF 1 delta, belongs to the EF-1-beta/EF-1-delta family. It is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. EEF1D is phosphorylated upon DNA damage, probably by ATM or ATR. The calculated molecular weight of EEF1D is a 31 kDa, but the modified protein is about 35-40 kDa. ( PMID: 21936567 )
Protocols
Product Specific Protocols | |
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WB protocol for EEF1D antibody 10630-1-AP | Download protocol |
IHC protocol for EEF1D antibody 10630-1-AP | Download protocol |
IP protocol for EEF1D antibody 10630-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Chem Biol A photoaffinity labeling strategy identified EF1A1 as a binding protein of cyclic dinucleotide 2'3'-cGAMP. | ||
EMBO Rep Transformation of eEF1Bδ into heat-shock response transcription factor by alternative splicing.
| ||
J Exp Clin Cancer Res EEF1D overexpression promotes osteosarcoma cell proliferation by facilitating Akt-mTOR and Akt-bad signaling.
| ||
Front Mol Biosci Heat Shock-Induced Dephosphorylation of Eukaryotic Elongation Factor 1BδL by Protein Phosphatase 1. | ||
Int J Biochem Cell Biol The alteration of protein profile induced by cigarette smoking via oxidative stress in mice epididymis. | ||
Proteomics Pulmonary pressure reduction attenuates expression of proteins identified by lung proteomic profiling in pulmonary hypertensive rats. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Wei (Verified Customer) (03-12-2020) | Sharp and strong band for western blot
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