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  • KD/KO Validated

EEF1D Polyclonal antibody

EEF1D Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 10630-1-AP


Antigen NY CO 4, EEF1D, EF 1 delta, EF 1D, EF1D, Elongation factor 1 delta, FP1047

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, U-87 MG cells, U2OS cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman pancreas cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10630-1-AP targets EEF1D in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EEF1D fusion protein Ag0983
Full Name eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC007847
Gene Symbol EEF1D
Gene ID (NCBI) 1936
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EEF1D, also named as EF1D and EF 1 delta, belongs to the EF-1-beta/EF-1-delta family. It is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. EEF1D is phosphorylated upon DNA damage, probably by ATM or ATR. The calculated molecular weight of EEF1D is a 31 kDa, but the modified protein is about 35-40 kDa. ( PMID: 21936567 )


Product Specific Protocols
WB protocol for EEF1D antibody 10630-1-APDownload protocol
IHC protocol for EEF1D antibody 10630-1-APDownload protocol
IP protocol for EEF1D antibody 10630-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Chem Biol

A photoaffinity labeling strategy identified EF1A1 as a binding protein of cyclic dinucleotide 2'3'-cGAMP.

Authors - Yingjie Hou


Transformation of eEF1Bδ into heat-shock response transcription factor by alternative splicing.

Authors - Kaitsuka Taku T
  • KD Validated

J Exp Clin Cancer Res

EEF1D overexpression promotes osteosarcoma cell proliferation by facilitating Akt-mTOR and Akt-bad signaling.

Authors - Dong-Dong Cheng
  • KD Validated

Front Mol Biosci

Heat Shock-Induced Dephosphorylation of Eukaryotic Elongation Factor 1BδL by Protein Phosphatase 1.

Authors - Taku Kaitsuka

Int J Biochem Cell Biol

The alteration of protein profile induced by cigarette smoking via oxidative stress in mice epididymis.

Authors - Zhu Zijue Z


Pulmonary pressure reduction attenuates expression of proteins identified by lung proteomic profiling in pulmonary hypertensive rats.

Authors - ?stergaard Louise L


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Wei (Verified Customer) (03-12-2020)

Sharp and strong band for western blot

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart