Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, U-87 MG cells, U2OS cells, NIH/3T3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human pancreas cancer tissue, human prostate cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10630-1-AP targets EEF1D in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0983 Product name: Recombinant human EEF1D protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 347-647 aa of BC007847 Sequence: SHRPGPRSGLSVSSLRPNRKMATNFLAHEKIWFDKFKYDDAERRFYEQMNGPVAGASRQENGASVILRDIARARENIQKSLAGSSGPGASSGTSGDHGELVVRIASLEVENQSLRGVVQELQQAISKLEARLNVLEKSSPGHRATAPQTQHVSPMRQVEPPAKKPATPAEDDEDDDIDLFGSDNEEEDKEAAQLREERLRQYAEKKAKKPALVAKSSILLDVKPWDDETDMAQLEACVRSIQLDGLVWGASKLVPVGYGIRKLQIQCVVEDDKVGTDLLEEEITKFEEHVQSVDIAAFNKI Predict reactive species |
| Full Name | eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) |
| Calculated Molecular Weight | 31 kDa |
| Observed Molecular Weight | 35-40 kDa |
| GenBank Accession Number | BC007847 |
| Gene Symbol | EEF1D |
| Gene ID (NCBI) | 1936 |
| RRID | AB_2230993 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P29692 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EEF1D, also named as EF1D and EF 1 delta, belongs to the EF-1-beta/EF-1-delta family. It is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. EEF1D is phosphorylated upon DNA damage, probably by ATM or ATR. The calculated molecular weight of EEF1D is a 31 kDa, but the modified protein is about 35-40 kDa. ( PMID: 21936567 )
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for EEF1D antibody 10630-1-AP | Download protocol |
| IP protocol for EEF1D antibody 10630-1-AP | Download protocol |
| WB protocol for EEF1D antibody 10630-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The EEF1D Polyclonal antibody (Cat# 10630-1-AP) has 13 citations published in journals including PNAS, EMBO Journal, EMBO Reports, Cell Chemical Biology, Journal of Experimental & Clinical Cancer Research, International Journal of Cancer, iScience, Frontiers in Molecular Biosciences, Proteomics, Journal of Virology, International Journal of Biochemistry & Cell Biology, and Life Science Alliance. Research fields span cancer biology (osteosarcoma, colorectal cancer), virology (HCMV, KSHV), autophagy, ER organization, heat-shock response, neuronal signaling, and proteomics.
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res EEF1D overexpression promotes osteosarcoma cell proliferation by facilitating Akt-mTOR and Akt-bad signaling.
| ||
Proc Natl Acad Sci U S A RNA-targeted proteomics identifies YBX1 as critical for efficient HCMV mRNA translation | ||
Cell Chem Biol A photoaffinity labeling strategy identified EF1A1 as a binding protein of cyclic dinucleotide 2'3'-cGAMP. | ||
EMBO J Autophagosomes anchor an AKAP11-dependent regulatory checkpoint that shapes neuronal PKA signaling | ||
EMBO Rep Transformation of eEF1Bδ into heat-shock response transcription factor by alternative splicing.
| ||
Int J Cancer EEF1D stabilized by SRSF9 promotes colorectal cancer via enhancing the proliferation and metastasis |
Reviews
What customers say
The product received a 5-star rating from a researcher who used it for Western Blot on mouse heart tissue. The reviewer reported obtaining a sharp and strong band, indicating excellent specificity and sensitivity for their application. Overall, the feedback highlights reliable performance in WB with clear, well-defined results.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Wei (Verified Customer) (03-12-2020) | Sharp and strong band for western blot
|
