|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human pancreas cancer tissue, human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10630-1-AP targets EEF1D in WB, IP, IHC, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||EEF1D fusion protein Ag0983|
|Full Name||eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)|
|Calculated molecular weight||31 kDa|
|Observed molecular weight||40 kDa|
|GenBank accession number||BC007847|
|Gene ID (NCBI)||1936|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
EEF1D, also named as EF1D and EF 1 delta, belongs to the EF-1-beta/EF-1-delta family. It is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. EEF1D is phosphorylated upon DNA damage, probably by ATM or ATR. The calculated molecular weight of EEF1D is a 31 kDa, but the modified protein is about 35-40 kDa. ( PMID: 21936567 )
Pulmonary pressure reduction attenuates expression of proteins identified by lung proteomic profiling in pulmonary hypertensive rats.
Int J Biochem Cell Biol
The alteration of protein profile induced by cigarette smoking via oxidative stress in mice epididymis.
Front Mol Biosci
Heat Shock-Induced Dephosphorylation of Eukaryotic Elongation Factor 1BδL by Protein Phosphatase 1.
J Exp Clin Cancer Res
EEF1D overexpression promotes osteosarcoma cell proliferation by facilitating Akt-mTOR and Akt-bad signaling.
Transformation of eEF1Bδ into heat-shock response transcription factor by alternative splicing.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Wei (Verified Customer) (03-12-2020)
Sharp and strong band for western blot