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  • KD/KO Validated

EEF1D Polyclonal antibody

EEF1D Polyclonal Antibody for IHC, IP,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 10630-1-AP


Antigen NY CO 4, EEF1D, EF 1 delta, EF 1D, EF1D, Elongation factor 1 delta, FP1047

Tested Applications

Positive IP detected inHeLa cells
Positive IHC detected inhuman pancreas cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10630-1-AP targets EEF1D in WB, IP, IHC, CoIP applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EEF1D fusion protein Ag0983
Full Name eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
Calculated molecular weight 31 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC007847
Gene symbol EEF1D
Gene ID (NCBI) 1936
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EEF1D, also named as EF1D and EF 1 delta, belongs to the EF-1-beta/EF-1-delta family. It is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. EEF1D is phosphorylated upon DNA damage, probably by ATM or ATR. The calculated molecular weight of EEF1D is a 31 kDa, but the modified protein is about 35-40 kDa. ( PMID: 21936567 )


Product Specific Protocols
IHC protocol for EEF1D antibody 10630-1-APDownload protocol
IP protocol for EEF1D antibody 10630-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Pulmonary pressure reduction attenuates expression of proteins identified by lung proteomic profiling in pulmonary hypertensive rats.

Authors - ?stergaard Louise L

Int J Biochem Cell Biol

The alteration of protein profile induced by cigarette smoking via oxidative stress in mice epididymis.

Authors - Zhu Zijue Z

Front Mol Biosci

Heat Shock-Induced Dephosphorylation of Eukaryotic Elongation Factor 1BδL by Protein Phosphatase 1.

Authors - Taku Kaitsuka

J Exp Clin Cancer Res

EEF1D overexpression promotes osteosarcoma cell proliferation by facilitating Akt-mTOR and Akt-bad signaling.

Authors - Dong-Dong Cheng
  • KD Validated


Transformation of eEF1Bδ into heat-shock response transcription factor by alternative splicing.

Authors - Kaitsuka Taku T
  • KD Validated

Cell Chem Biol

A photoaffinity labeling strategy identified EF1A1 as a binding protein of cyclic dinucleotide 2'3'-cGAMP.

Authors - Yingjie Hou


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Wei (Verified Customer) (03-12-2020)

Sharp and strong band for western blot

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart