• Featured Product
  • KD/KO Validated

EEF1D Polyclonal antibody

EEF1D Polyclonal Antibody for IHC, IP,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, CoIP,ELISA

Conjugate

Unconjugated

Cat no : 10630-1-AP

Synonyms

Antigen NY CO 4, EEF1D, EF 1 delta, EF 1D, EF1D, Elongation factor 1 delta, FP1047



Tested Applications

Positive IP detected inHeLa cells
Positive IHC detected inhuman pancreas cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10630-1-AP targets EEF1D in WB, IP, IHC, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EEF1D fusion protein Ag0983
Full Name eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
Calculated molecular weight 31 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC007847
Gene symbol EEF1D
Gene ID (NCBI) 1936
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EEF1D, also named as EF1D and EF 1 delta, belongs to the EF-1-beta/EF-1-delta family. It is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. EEF1D is phosphorylated upon DNA damage, probably by ATM or ATR. The calculated molecular weight of EEF1D is a 31 kDa, but the modified protein is about 35-40 kDa. ( PMID: 21936567 )

Protocols

Product Specific Protocols
IHC protocol for EEF1D antibody 10630-1-APDownload protocol
IP protocol for EEF1D antibody 10630-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Proteomics

Pulmonary pressure reduction attenuates expression of proteins identified by lung proteomic profiling in pulmonary hypertensive rats.

Authors - ?stergaard Louise L
mouseWB

Int J Biochem Cell Biol

The alteration of protein profile induced by cigarette smoking via oxidative stress in mice epididymis.

Authors - Zhu Zijue Z
mouseWB,CoIP

Front Mol Biosci

Heat Shock-Induced Dephosphorylation of Eukaryotic Elongation Factor 1BδL by Protein Phosphatase 1.

Authors - Taku Kaitsuka
humanWB

J Exp Clin Cancer Res

EEF1D overexpression promotes osteosarcoma cell proliferation by facilitating Akt-mTOR and Akt-bad signaling.

Authors - Dong-Dong Cheng
  • KD Validated
human,mouseWB

EMBO Rep

Transformation of eEF1Bδ into heat-shock response transcription factor by alternative splicing.

Authors - Kaitsuka Taku T
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Wei (Verified Customer) (03-12-2020)

Sharp and strong band for western blot

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart