eEF2K Polyclonal antibody

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• eEF2K Polyclonal antibody (13510-1-AP)

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• KD/KO Validated

eEF2K Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 13510-1-AP Copied!

Publications (10)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

eEF-2K, eEF-2 kinase, eEF 2K, eEF 2 kinase, EC:2.7.11.20

• 

  WB analysis of HEK-293 using 13510-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13510-1-AP (EEF2K Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 13510-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13510-1-AP (EEF2K Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of PC-3 using 13510-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13510-1-AP (EEF2K Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of PC-3 using 13510-1-AP

  IP result of anti-eEF2K (IP:13510-1-AP, 4ug; Detection:13510-1-AP 1:500) with PC-3 cells lysate 3200ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of PC-3 using 13510-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 13510-1-AP (EEF2K antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: PC-3 cells, Jurkat cells, HEK-293 cells
• Positive IP detected in: PC-3 cells
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13510-1-AP targets eEF2K in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4432

  Product name: Recombinant human EEF2K protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 376-725 aa of BC032665

  Sequence: LSENSGDENMSDVTFDSLPSSPSSATPHSQKLDHLHWPVFSDLDNMASRDHDHLDNHRESENSGDSGYPSEKRGELDDPEPREHGHSYSNRKYESDEDSLGSSGRVCVEKWNLLNSSRLHLPRASAVALEVQRLNALDLEKKIGKSILGKVHLAMVRYHEGGRFCEKGEEWDQESAVFHLEHAANLGELEAIVGLGLMYSQLPHHILADVSLKETEENKTKGFDYLLKAAEAGDRQSMILVARAFDSGQNLSPDRCQDWLEALHWYNTALEMTDCDEGGEYDGMQDEPRYMMLAREAEMLFTGGYGLEKDPQRSGDLYTQAAEAAMEAMKGRLANQYYQKAEEAWAQMEE

• Full Name: eukaryotic elongation factor-2 kinase
• Calculated Molecular Weight: 725 aa, 100 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC032665
• Gene Symbol: EEF2K
• Gene ID (NCBI): 29904
• RRID: AB_2277761
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O00418
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The eEF2K antibody (Cat# 13510-1-AP) has 10 total citations published in journals including Cell Metabolism, Cancer Letters, Journal of Medicinal Chemistry, Cells, International Journal of Molecular Sciences, Journal of Molecular Medicine, Medical Oncology, JOR Spine, Journal of Biomedical Research, and Oncology Letters. Research fields span AMPK/mTOR signaling, autophagy, cancer biology (triple-negative breast cancer, radiation sensitivity), drug repurposing, fibrosis, intervertebral disc degeneration, and meiotic spindle organization.

Automatically generated from the 10 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human,mouse	WB	Cell Metab; AMPKα2 signals amino acid insufficiency to inhibit protein synthesis.; Authors - Yunzi Mao
human	WB	Cancer Lett; eEF-2 Kinase-targeted miR-449b confers radiation sensitivity to cancer cells.; Authors - Cheng Ji
		J Med Chem; Fluoxetine-Conjugated Platinum(IV) Prodrugs Targeting eEF2K and Conquering Multidrug Resistance against Triple-Negative Breast Cancer; Authors - Jia-Qian Wang
rat	WB,IP,CoIP	Cells; SIRT1 Protects the Heart from ER Stress-Induced Injury by Promoting eEF2K/eEF2-Dependent Autophagy.; Authors - Julie Pires Da Silva ; KD Validated
mouse	WB,IHC	Int J Mol Sci; Integrative Transcriptomic Analysis Identifies Shared Immune-Fibrotic Transcriptional Programs Across Crohn's Disease and Idiopathic Pulmonary Fibrosis.; Authors - Renwei Luo
		J Mol Med (Berl); FOXM1 plays a role in autophagy by transcriptionally regulating Beclin-1 and LC3 genes in human triple-negative breast cancer cells.; Authors - Zuhal Hamurcu