EFCAB4A Polyclonal antibody

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• EFCAB4A Polyclonal antibody (32115-1-AP)

• 

  WB analysis using 32115-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32115-1-AP (EFCAB4A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 32115-1-AP

  WB result of EFCAB4A antibody (32115-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EFCAB4A transfected LNCaP cells.

• Featured Product
• KD/KO Validated

EFCAB4A Polyclonal Antibody for WB, ELISA

Cat No. 32115-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Ca2+ Release-Activated Ca2+ (CRAC) Channel Regulator 2B, Calcium Release Activated Channel Regulator 2B, Calcium release-activated calcium channel regulator 2B, Calcium release-activated channel regulator 2B, CRAC channel regulator 2B

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Tested Applications

• Positive WB detected in: LNCaP cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32115-1-AP targets EFCAB4A in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag37031

  Product name: Recombinant human EFCAB4A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 100-255 aa of BC033196

  Sequence: SAQGANPCRTPEETFESGGLDVQGTAGSLDEEEEEEERFHTVLEQLGVAPVLGEQRAVRTLWARLQRERPELLGSFEDVLIRASACLEEAARERDGLEQALRRRESEHEREVRALYEETEQLREQSRRPPSQNFARGERRSRLELELQIREQDLER

• Full Name: EF-hand calcium binding domain 4A
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC033196
• Gene Symbol: EFCAB4A
• Gene ID (NCBI): 283229
• RRID: AB_3742528
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q8N4Y2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EFCAB4A (EF-hand calcium-binding domain-containing protein 4A), also known as CRACR2B (calcium release-activated calcium channel regulator 2B), is a calcium-binding protein containing EF-hand structural domain. EFCAB4A is thought to play a key role in calcium bank-manipulated calcium endocytosis (SOCE) in T cells by regulating activation of CRAC (calcium release-activated calcium channel) channels. This protein may perform a similar function to EFCAB4B, which acts as a cytoplasmic calcium sensor that forms a complex with ORAI1 and STIM1 in the region of the junction between the endoplasmic reticulum and the plasma membrane. The EFCAB4A protein contains two EF-hand structural domains, which may be involved in calcium bank-manipulated calcium endocytosis.